Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tetrachlorohydroquinone | activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone | Sphingobium chlorophenolicum |
Cloned (Comment) | Organism |
---|---|
expression in Eshcerichia coli | Sphingobium chlorophenolicum |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN | Sphingobium chlorophenolicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pentachlorophenol | competitive, 55% loss of activity at 0.1 mM | Sphingobium chlorophenolicum | |
tetrachlorohydroquinone | activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone | Sphingobium chlorophenolicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | protein contains 1.7 mol of iron per mol | Sphingobium chlorophenolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
protein exists as trimer, dynamic light scattering | Sphingobium chlorophenolicum |
36000 | - |
- |
Sphingobium chlorophenolicum |
36500 | - |
- |
Sphingobium chlorophenolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | Q47914 | - |
- |
Sphingobium chlorophenolicum ATCC 39723 | Q47914 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein,. Purified enzyme is colorless | Sphingobium chlorophenolicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
pH 7.0, 25°C | Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
? | |
2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
Sphingobium chlorophenolicum ATCC 39723 | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated | Sphingobium chlorophenolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Sphingobium chlorophenolicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
70% of maximum activity | Sphingobium chlorophenolicum |
8 | - |
20% of maximum activity | Sphingobium chlorophenolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster | Sphingobium chlorophenolicum | |
[2Fe-2S]-center | the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster | Sphingobium chlorophenolicum |