Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-aspartate | the enzyme is activated at 5 mM L-aspartate | Leishmania mexicana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells | Leishmania mexicana |
expressed in Escherichia coli Rosetta (DE3) pLysS cells | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania mexicana | |
0.04 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania major | |
0.44 | - |
(S)-malate | at pH 7.4 and 37°C | Leishmania major | |
0.65 | - |
(S)-malate | at pH 7.4 and 37°C | Leishmania mexicana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.5 mM used in assay conditions, Km value of 0.0092 mM | Leishmania major | |
Mn2+ | 0.5 mM used in assay conditions, Km value of 0.02 mM | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Leishmania mexicana | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Leishmania major | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Leishmania mexicana | - |
(S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Leishmania major | - |
(S)-malate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Leishmania mexicana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephaycryl S200 gel filtration | Leishmania major |
Ni-NTA column chromatography, and Sephaycryl S200 gel filtration | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Leishmania mexicana | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Leishmania major | pyruvate + CO2 + NADPH + H+ | - |
r | |
additional information | no activity with NAD+ | Leishmania mexicana | ? | - |
- |
|
additional information | no activity with NAD+ | Leishmania major | ? | - |
- |
|
pyruvate + CO2 + NADPH + H+ | - |
Leishmania mexicana | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Leishmania major | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Leishmania mexicana |
? | x * 65000, SDS-PAGE | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
m-NADP-ME | - |
Leishmania mexicana |
m-NADP-ME | - |
Leishmania major |
ME-61 | - |
Leishmania mexicana |
ME-70 | - |
Leishmania major |
NADP+-dependent ME | - |
Leishmania mexicana |
NADP+-dependent ME | - |
Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
106.93 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania mexicana | |
113.63 | - |
(S)-malate | at pH 7.4 and 37°C | Leishmania major | |
119.89 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania major | |
122.23 | - |
(S)-malate | at pH 7.4 and 37°C | Leishmania mexicana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | the enzyme exhibits the highest catalytic performance at a relatively narrow pH range (7.0-8.0) | Leishmania mexicana |
7 | 8 | the enzyme exhibits the highest catalytic performance at a relatively narrow pH range (7.0-8.0) | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leishmania mexicana | |
NADP+ | - |
Leishmania major |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leishmania mexicana | calculated from amino acid sequence | - |
5.8 |
Leishmania major | calculated from amino acid sequence | - |
8.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
188 | - |
(S)-malate | at pH 7.4 and 37°C | Leishmania mexicana | |
258 | - |
(S)-malate | at pH 7.4 and 37°C | Leishmania major | |
3000 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania major | |
4650 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania mexicana |