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Literature summary for 1.1.1.40 extracted from

  • Giordana, L.; Sosa, M.; Leroux, A.; Mendoza, E.; Petray, P.; Nowicki, C.
    Molecular and functional characterization of two malic enzymes from Leishmania parasites (2018), Mol. Biochem. Parasitol., 219, 67-76 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-aspartate the enzyme is activated at 5 mM L-aspartate Leishmania mexicana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells Leishmania mexicana
expressed in Escherichia coli Rosetta (DE3) pLysS cells Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
NADP+ at pH 7.4 and 37°C Leishmania mexicana
0.04
-
NADP+ at pH 7.4 and 37°C Leishmania major
0.44
-
(S)-malate at pH 7.4 and 37°C Leishmania major
0.65
-
(S)-malate at pH 7.4 and 37°C Leishmania mexicana

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mM used in assay conditions, Km value of 0.0092 mM Leishmania major
Mn2+ 0.5 mM used in assay conditions, Km value of 0.02 mM Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Leishmania mexicana
-
pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+ Leishmania major
-
pyruvate + CO2 + NADPH + H+
-
r
pyruvate + CO2 + NADPH + H+ Leishmania mexicana
-
(S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+ Leishmania major
-
(S)-malate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-
Leishmania mexicana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephaycryl S200 gel filtration Leishmania major
Ni-NTA column chromatography, and Sephaycryl S200 gel filtration Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Leishmania mexicana pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+
-
Leishmania major pyruvate + CO2 + NADPH + H+
-
r
additional information no activity with NAD+ Leishmania mexicana ?
-
-
additional information no activity with NAD+ Leishmania major ?
-
-
pyruvate + CO2 + NADPH + H+
-
Leishmania mexicana (S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+
-
Leishmania major (S)-malate + NADP+
-
r

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Leishmania mexicana
? x * 65000, SDS-PAGE Leishmania major

Synonyms

Synonyms Comment Organism
m-NADP-ME
-
Leishmania mexicana
m-NADP-ME
-
Leishmania major
ME-61
-
Leishmania mexicana
ME-70
-
Leishmania major
NADP+-dependent ME
-
Leishmania mexicana
NADP+-dependent ME
-
Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
106.93
-
NADP+ at pH 7.4 and 37°C Leishmania mexicana
113.63
-
(S)-malate at pH 7.4 and 37°C Leishmania major
119.89
-
NADP+ at pH 7.4 and 37°C Leishmania major
122.23
-
(S)-malate at pH 7.4 and 37°C Leishmania mexicana

pH Range

pH Minimum pH Maximum Comment Organism
7 8 the enzyme exhibits the highest catalytic performance at a relatively narrow pH range (7.0-8.0) Leishmania mexicana
7 8 the enzyme exhibits the highest catalytic performance at a relatively narrow pH range (7.0-8.0) Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Leishmania mexicana
NADP+
-
Leishmania major

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania mexicana calculated from amino acid sequence
-
5.8
Leishmania major calculated from amino acid sequence
-
8.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
188
-
(S)-malate at pH 7.4 and 37°C Leishmania mexicana
258
-
(S)-malate at pH 7.4 and 37°C Leishmania major
3000
-
NADP+ at pH 7.4 and 37°C Leishmania major
4650
-
NADP+ at pH 7.4 and 37°C Leishmania mexicana