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Literature summary for 1.1.1.40 extracted from

  • Hernandez, M.A.; Alvarez, H.M.
    Increasing lipid production using an NADP+-dependent malic enzyme from Rhodococcus jostii (2019), Microbiology, 165, 4-14 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus jostii

Inhibitors

Inhibitors Comment Organism Structure
sesamol
-
Rhodococcus fascians
sesamol
-
Rhodococcus jostii
sesamol
-
Rhodococcus opacus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rhodococcus opacus 5829
-
cytosol
-
Rhodococcus fascians 5829
-
cytosol
-
Rhodococcus jostii 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Rhodococcus opacus
Mg2+ 10 mM used in assay conditions Rhodococcus fascians
Mg2+ 10 mM used in assay conditions Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Rhodococcus opacus
-
pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+ Rhodococcus fascians
-
pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+ Rhodococcus jostii
-
pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+ Rhodococcus opacus PD630
-
pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+ Rhodococcus fascians F7
-
pyruvate + CO2 + NADPH + H+
-
r
pyruvate + CO2 + NADPH + H+ Rhodococcus opacus
-
(S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+ Rhodococcus fascians
-
(S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+ Rhodococcus jostii
-
(S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+ Rhodococcus opacus PD630
-
(S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+ Rhodococcus fascians F7
-
(S)-malate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Rhodococcus fascians
-
-
-
Rhodococcus fascians F7
-
-
-
Rhodococcus jostii
-
-
-
Rhodococcus opacus
-
-
-
Rhodococcus opacus PD630
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0019
-
in the presence of 0.1 g/l ammonium chloride, at pH 7.5 and 30°C Rhodococcus fascians
0.0021
-
in the presence of 1.0 g/l ammonium chloride, at pH 7.5 and 30°C Rhodococcus fascians
0.0023
-
in the absence of ammonium chloride, at pH 7.5 and 30°C Rhodococcus fascians
0.0134
-
in the presence of 1.0 g/l ammonium chloride and 1.5 mM sesamol, at pH 7.5 and 30°C Rhodococcus jostii
0.0217
-
in the presence of 0.1 g/l ammonium chloride and 1.5 mM sesamol, at pH 7.5 and 30°C Rhodococcus jostii
0.0225
-
in the absence of ammonium chloride and in the presence of 1.5 mM sesamol, at pH 7.5 and 30°C Rhodococcus jostii
0.0234
-
in the presence of 1.0 g/l ammonium chloride, at pH 7.5 and 30°C Rhodococcus jostii
0.0342
-
in the absence of ammonium chloride, at pH 7.5 and 30°C Rhodococcus jostii
0.0435
-
in the presence of 0.1 g/l ammonium chloride, at pH 7.5 and 30°C Rhodococcus jostii
0.059
-
in the presence of 1.0 g/l ammonium chloride, at pH 7.5 and 30°C Rhodococcus opacus
0.1343
-
in the absence of ammonium chloride, at pH 7.5 and 30°C Rhodococcus opacus
0.1533
-
in the presence of 0.1 g/l ammonium chloride, at pH 7.5 and 30°C Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Rhodococcus opacus pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+
-
Rhodococcus fascians pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+
-
Rhodococcus jostii pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+
-
Rhodococcus opacus PD630 pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+
-
Rhodococcus fascians F7 pyruvate + CO2 + NADPH + H+
-
r
additional information no activity with NAD+ Rhodococcus opacus ?
-
-
additional information no activity with NAD+ Rhodococcus fascians ?
-
-
additional information no activity with NAD+ Rhodococcus jostii ?
-
-
additional information no activity with NAD+ Rhodococcus opacus PD630 ?
-
-
additional information no activity with NAD+ Rhodococcus fascians F7 ?
-
-
pyruvate + CO2 + NADPH + H+
-
Rhodococcus opacus (S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+
-
Rhodococcus fascians (S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+
-
Rhodococcus jostii (S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+
-
Rhodococcus opacus PD630 (S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+
-
Rhodococcus fascians F7 (S)-malate + NADP+
-
r

Synonyms

Synonyms Comment Organism
NADP+-dependent malic enzyme
-
Rhodococcus opacus
NADP+-dependent malic enzyme
-
Rhodococcus fascians
NADP+-dependent malic enzyme
-
Rhodococcus jostii
NADP+-ME
-
Rhodococcus opacus
NADP+-ME
-
Rhodococcus fascians
NADP+-ME
-
Rhodococcus jostii
RHA1_RS44255
-
Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme prefers NADP+ as a coenzyme Rhodococcus opacus
NADP+ the enzyme prefers NADP+ as a coenzyme Rhodococcus fascians
NADP+ the enzyme prefers NADP+ as a coenzyme Rhodococcus jostii

Expression

Organism Comment Expression
Rhodococcus jostii total enzyme activity increases by 1.8fold during cultivation under nitrogen-limiting conditions up
Rhodococcus opacus total enzyme activity increases by 2.6fold during cultivation under nitrogen-limiting conditions up