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Literature summary for 1.1.1.40 extracted from

  • Guagliardi, A.; Manco, G.; Rossi, M.; Bartolucci, S.
    Stability and activity of a thermostable malic enzyme in denaturants and water-miscible organic solvents (1989), Eur. J. Biochem., 183, 25-30.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methanol activation is reversible and inversely related to the temperature Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
NADP+ pH 7.0, 60°C Saccharolobus solfataricus
0.071
-
(S)-malate pH 7.0, 60°C Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
dimethylformamide 25°C, after a 24-h incubation in 50% dimethylformamide, the enzyme is completely active Saccharolobus solfataricus
Ethanol 15%, 25°C, 24 h, 15% loss of activity. Half-life is 8 h in 50% ethanol Saccharolobus solfataricus
guanidine-HCl 25°C, half-life is 30 min in 6 M guanidine hydrochloride Saccharolobus solfataricus
Methanol 50%, 25°C, 24 h, 15% loss of activity. Half-life of 50% methanol is 16 h at 45°C and 3 min at 60°C Saccharolobus solfataricus
SDS 25°C, the enzyme is not inactivated in 0.05% SDS over 24 h. Half-life is 5 h in 0.075% SDS Saccharolobus solfataricus
tetrahydrofuran half-life is 30 min in 50% tetrahydrofuran Saccharolobus solfataricus
urea 25°C, the enzyme is not inactivated in 4 M urea over 24 h. Half-life is 10 h in 7.5 M urea Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Saccharolobus solfataricus pyruvate + CO2 + NADPH
-
?
(S)-malate + NADP+
-
Saccharolobus solfataricus MT-4 / DSM 5833 pyruvate + CO2 + NADPH
-
?

Synonyms

Synonyms Comment Organism
malic enzyme
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
12 h, in absence of solvent, stable Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Saccharolobus solfataricus