Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methanol | activation is reversible and inversely related to the temperature | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
NADP+ | pH 7.0, 60°C | Saccharolobus solfataricus | |
0.071 | - |
(S)-malate | pH 7.0, 60°C | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
dimethylformamide | 25°C, after a 24-h incubation in 50% dimethylformamide, the enzyme is completely active | Saccharolobus solfataricus |
Ethanol | 15%, 25°C, 24 h, 15% loss of activity. Half-life is 8 h in 50% ethanol | Saccharolobus solfataricus |
guanidine-HCl | 25°C, half-life is 30 min in 6 M guanidine hydrochloride | Saccharolobus solfataricus |
Methanol | 50%, 25°C, 24 h, 15% loss of activity. Half-life of 50% methanol is 16 h at 45°C and 3 min at 60°C | Saccharolobus solfataricus |
SDS | 25°C, the enzyme is not inactivated in 0.05% SDS over 24 h. Half-life is 5 h in 0.075% SDS | Saccharolobus solfataricus |
tetrahydrofuran | half-life is 30 min in 50% tetrahydrofuran | Saccharolobus solfataricus |
urea | 25°C, the enzyme is not inactivated in 4 M urea over 24 h. Half-life is 10 h in 7.5 M urea | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Saccharolobus solfataricus | pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | pyruvate + CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
12 h, in absence of solvent, stable | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharolobus solfataricus |