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Literature summary for 1.1.1.40 extracted from
- Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure (2007), J. Bacteriol., 189, 5937-5946.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloning in strain K-12, expression of the His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates at up to 4 mM, inhibition above, probably due to blockage of substrate binding, Km is 0.19 mM | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | with malate as the variable substrate the kinetics are nonhyperbolic, exhibiting a sigmoidal response with a positive Hill coefficient | Escherichia coli | |
| 0.0415 | - |
NADP+ | pH 7.5, recombinant MaeB | Escherichia coli | |
| 3.41 | - |
(S)-malate | pH 7.5, recombinant MaeB | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates at 2 mM | Escherichia coli | |
| Mg2+ | activates at up to 4 mM, inhibition above, probably due to blockage of substrate binding | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Escherichia coli | MEs are essential enzymes for growth on TCA cycle intermediates or on substrates that enter central metabolism via acetyl-coenzyme A, the reaction plays a role in the C4 metabolism, regulation, overview | pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene maeB or ypfF, several isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged MaeB from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | MEs are essential enzymes for growth on TCA cycle intermediates or on substrates that enter central metabolism via acetyl-coenzyme A, the reaction plays a role in the C4 metabolism, regulation, overview | Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | MaeB possesses a multimodular structure, it is composed of two distinct domains, MWs of the different isozymes, overview | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MaeB | - |
Escherichia coli |
| NADP-specific ME | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 66.6 | - |
NADP+ | pH 7.5, recombinant MaeB | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reverse reaction | Escherichia coli |
| 7.5 | - |
forward reaction | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity with NAD+ | Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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