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Literature summary for 1.1.1.40 extracted from
- A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis (2007), Biotechnol. Lett., 29, 1129-1134.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is induced by salt stress, NADP-ME activities in seedlings, leaves, and roots increase in response to NaCl | Oryza sativa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Arabidopsis thaliana under control of the 35S promoter | Oryza sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transgenic Arabidopsis thaliana plants overexpressing rice cytosolic NADP-ME have a greater salt tolerance at the seedling stage than wild-type plants in MS medium-supplemented with different levels of NaCl, no obvious morphological or developmental differences between the transgenic and wild-type plants | Oryza sativa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | cytoNADPME | Oryza sativa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Oryza sativa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Oryza sativa | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q6SZS7 | var. indica, cv. Nipponbare | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
| root | - |
Oryza sativa | - |
| seedling | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
root enzyme under salt stress after 24 h | Oryza sativa |
| 0.2 | - |
leaf enzyme under salt stress after 24 h | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Oryza sativa | pyruvate + CO2 + NADPH | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytoNADPME | - |
Oryza sativa |
| NADP-malic enzyme | - |
Oryza sativa |
| NADP-ME | - |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, forward reaction | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Oryza sativa | |
| NADPH | - |
Oryza sativa | |
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