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Literature summary for 1.1.1.40 extracted from
- Carbon metabolism in germinating Ricinus communis cotyledons. Purification, characterization and developmental profile of NADP-dependent malic enzyme (1997), Physiol. Plant., 101, 821-826.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | activates | Ricinus communis |  |
| CoA | activates | Ricinus communis | |
| palmitoyl-CoA | activates | Ricinus communis | |
| succinate | activates | Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0082 | - |
NADP+ | - |
Ricinus communis | |
| 0.68 | - |
L-malate | - |
Ricinus communis | |
| 8.2 | - |
NADP+ | - |
Ricinus communis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required | Ricinus communis | |
| Mn2+ | Mn2+ or Mg2+ required | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
4 * 72000, SDS-PAGE | Ricinus communis |
| 285000 | - |
gel filtration | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | reaction velocity is 29times higher in the direction of decarboxylation than in the direction of the carboxylation | Ricinus communis | pyruvate + CO2 + NADPH | - |
r | |
| pyruvate + CO2 + NADPH | reaction velocity is 29times higher in the direction of decarboxylation than in the direction of the carboxylation | Ricinus communis | L-malate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 72000, SDS-PAGE | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 7.2 | - |
Ricinus communis |
| 7.1 | - |
- |
Ricinus communis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | pH 6.5: about 55% of maximal activity, pH 7.5: about 35% of maximal activity | Ricinus communis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | cofactor | Ricinus communis | |
| NADPH | cofactor | Ricinus communis | |
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Release 2023.1 (January 2023)
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