Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity | Bacillus thuringiensis serovar kurstaki |
Cloned (Comment) | Organism |
---|---|
gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Bacillus thuringiensis serovar kurstaki |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Bacillus thuringiensis serovar kurstaki | |
additional information | nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity | Bacillus thuringiensis serovar kurstaki |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
(2R,3R)-butane-2,3-diol | pH 10.0, 50°C, recombinant enzyme | Bacillus thuringiensis serovar kurstaki | |
3.67 | - |
(2R,3S)-butane-2,3-diol | pH 10.0, 50°C, recombinant enzyme | Bacillus thuringiensis serovar kurstaki |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, the enzyme has a catalytic Zn site (Cys37, His69 and Glu151) and a structural Zn site (Cys99, Cys102, Cys105 and Cys113) | Bacillus thuringiensis serovar kurstaki |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki | - |
(3R)-acetoin + NADH + H+ | - |
r | |
(2R,3R)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki ACCC 10066 | - |
(3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki | - |
(3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki ACCC 10066 | - |
(3R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis serovar kurstaki | A0A0F6FX23 | - |
- |
Bacillus thuringiensis serovar kurstaki ACCC 10066 | A0A0F6FX23 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ion exchange chromatography | Bacillus thuringiensis serovar kurstaki |
Storage Stability | Organism |
---|---|
4°C, BtBDH shows good stability after storage for 3 months | Bacillus thuringiensis serovar kurstaki |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3R)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki ACCC 10066 | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | slightly preferred substrate | Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki ACCC 10066 | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | slightly preferred substrate | Bacillus thuringiensis serovar kurstaki ACCC 10066 | (3R)-acetoin + NADH + H+ | - |
r | |
additional information | enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl | Bacillus thuringiensis serovar kurstaki | ? | - |
- |
|
additional information | enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl | Bacillus thuringiensis serovar kurstaki ACCC 10066 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 37800, about, sequence calculation, x * 40000, recombinant enzyme, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
Synonyms | Comment | Organism |
---|---|---|
(2R,3R)-2,3-butanediol dehydrogenase | - |
Bacillus thuringiensis serovar kurstaki |
BtBDH | - |
Bacillus thuringiensis serovar kurstaki |
butanediol dehydrogenase | - |
Bacillus thuringiensis serovar kurstaki |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
reduction activity | Bacillus thuringiensis serovar kurstaki |
50 | - |
oxidation activity | Bacillus thuringiensis serovar kurstaki |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable up to | Bacillus thuringiensis serovar kurstaki |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction activity | Bacillus thuringiensis serovar kurstaki |
10 | - |
oxidation activity | Bacillus thuringiensis serovar kurstaki |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | stable at | Bacillus thuringiensis serovar kurstaki |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus thuringiensis serovar kurstaki | |
NADH | - |
Bacillus thuringiensis serovar kurstaki |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus thuringiensis serovar kurstaki | sequence calculation | - |
5.5 |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme BtBDH contains a GroES-like domain at the N terminus and a NAD(P)-binding domain at the C-terminus. Phylogenetic tree analysis reveals that BtBDH is a member ofthe (2R,3R)-2,3-BDH group. BtBDH has the typical (2R,3R)-2,3-butanediol dehydrogenase properties and belongs to the MDR superfamily. According to previous reports, (2R,3R)-2,3-BDH generally belongs to the MDR family, while meso-2,3-BDH is commonly clustered in the SDR (short chain dehydrogenase/reductase) family | Bacillus thuringiensis serovar kurstaki |