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show all sequences of 1.1.1.399

Isolation, purification and characterisation of 2-oxoglutarate reductase from Fusobacterium nucleatum

Gharbia, S.; Shah, H.; FEMS Microbiol. Lett. 80, 283-288 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl disulfide
-
Fusobacterium nucleatum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000004
-
NADH
at pH 7.5 and 25°C
Fusobacterium nucleatum
0.000064
-
2-oxoglutarate
at pH 7.5 and 25°C
Fusobacterium nucleatum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
x * 68000, SDS-PAGE
Fusobacterium nucleatum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-2-hydroxyglutarate + NAD+
Fusobacterium nucleatum
-
2-oxoglutarate + NADH + H+
-
-
r
2-oxoglutarate + NADH + H+
Fusobacterium nucleatum
-
(R)-2-hydroxyglutarate + NAD+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fusobacterium nucleatum
-
-
-
Purification (Commentary)
Commentary
Organism
Sepharose-4B-(glutathione-2-pyridyl disulfide) column chromatography
Fusobacterium nucleatum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-2-hydroxyglutarate + NAD+
-
738269
Fusobacterium nucleatum
2-oxoglutarate + NADH + H+
-
-
-
r
2-oxoglutarate + NADH + H+
-
738269
Fusobacterium nucleatum
(R)-2-hydroxyglutarate + NAD+
-
-
-
r
additional information
no activity with oxaloacetate, pyruvate, 2-oxobutyrate and glyoxylate
738269
Fusobacterium nucleatum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 68000, SDS-PAGE
Fusobacterium nucleatum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
60
the enzyme is stable at 45°C for 10 min, 70% of its activity is lost at 50°C, 5% of the original activity is retained at 55°C, while incubation at 60°C abolishes all activity
Fusobacterium nucleatum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
the enzyme is almost entirely specific towards NADH
Fusobacterium nucleatum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
the enzyme is almost entirely specific towards NADH
Fusobacterium nucleatum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl disulfide
-
Fusobacterium nucleatum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000004
-
NADH
at pH 7.5 and 25°C
Fusobacterium nucleatum
0.000064
-
2-oxoglutarate
at pH 7.5 and 25°C
Fusobacterium nucleatum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
x * 68000, SDS-PAGE
Fusobacterium nucleatum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-2-hydroxyglutarate + NAD+
Fusobacterium nucleatum
-
2-oxoglutarate + NADH + H+
-
-
r
2-oxoglutarate + NADH + H+
Fusobacterium nucleatum
-
(R)-2-hydroxyglutarate + NAD+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
Sepharose-4B-(glutathione-2-pyridyl disulfide) column chromatography
Fusobacterium nucleatum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-2-hydroxyglutarate + NAD+
-
738269
Fusobacterium nucleatum
2-oxoglutarate + NADH + H+
-
-
-
r
2-oxoglutarate + NADH + H+
-
738269
Fusobacterium nucleatum
(R)-2-hydroxyglutarate + NAD+
-
-
-
r
additional information
no activity with oxaloacetate, pyruvate, 2-oxobutyrate and glyoxylate
738269
Fusobacterium nucleatum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 68000, SDS-PAGE
Fusobacterium nucleatum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
60
the enzyme is stable at 45°C for 10 min, 70% of its activity is lost at 50°C, 5% of the original activity is retained at 55°C, while incubation at 60°C abolishes all activity
Fusobacterium nucleatum
Other publictions for EC 1.1.1.399
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287546
Zhao
A novel alpha-ketoglutarate re ...
Escherichia coli
J. Bacteriol.
178
232-239
1996
-
-
1
-
-
-
4
1
-
-
1
2
-
1
-
-
1
-
-
-
2
-
2
1
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-
1
1
-
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3
-
1
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-
1
-
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1
4
3
1
-
-
1
2
-
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-
1
-
-
2
-
2
1
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-
1
1
-
-
-
-
-
-
-
-
1
1
738269
Gharbia
Isolation, purification and ch ...
Fusobacterium nucleatum
FEMS Microbiol. Lett.
80
283-288
1991
-
-
-
-
-
-
1
2
-
-
1
2
-
2
-
-
1
-
-
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-
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3
1
-
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1
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-
1
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1
-
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-
1
-
2
-
-
1
2
-
-
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1
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-
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3
1
-
-
1
-
-
-
-
-
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-
-
-
-
-
738853
Gharbia
Identification of Fusobacteriu ...
Eubacterium sulci, Filifactor alocis, Fusobacterium gonidiaformans, Fusobacterium mortiferum, Fusobacterium naviforme, Fusobacterium necrogenes, Fusobacterium necrophorum, Fusobacterium nucleatum, Fusobacterium periodonticum, Fusobacterium russii, Fusobacterium simiae, Fusobacterium ulcerans, Fusobacterium varium
J. Med. Microbiol.
33
183-188
1990
-
-
-
-
-
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26
-
21
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26
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26
-
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26
-
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-
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-
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-
-
-
-
738426
Gharbia
-
Glutamate dehydrogenase and 2- ...
Fusobacterium nucleatum
Int. J. Syst. Bacteriol.
39
467-470
1989
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-
-
-
-
-
-
-
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2
-
1
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2
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2
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2
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738484
Lerud
Purification and properties of ...
Micrococcus aerogenes 228, Micrococcus aerogenes
J. Bacteriol.
106
571-577
1971
1
-
-
-
-
-
1
4
-
-
1
4
-
2
-
-
1
-
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-
-
2
8
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
4
-
-
1
4
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-
-
1
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-
2
8
1
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