BRENDA - Enzyme Database
show all sequences of 1.1.1.398

Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45

Van Hylckama Vlieg, J.; Kingma, J.; Kruizinga, W.; Janssen, D.; J. Bacteriol. 181, 2094-2101 (1999) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
NAD+
at pH 10.0 and 30°C
Rhodococcus sp. AD45
1.4
-
1-hydroxy-2-glutathionyl-2-methyl-3-butene
at pH 10.0 and 30°C
Rhodococcus sp. AD45
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
2 * 25000, SDS-PAGE
Rhodococcus sp. AD45
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus sp. AD45
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration
Rhodococcus sp. AD45
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.9
-
crude extract, at pH 10.0 and 30°C
Rhodococcus sp. AD45
18
-
after 6.2fold purification, at pH 10.0 and 30°C
Rhodococcus sp. AD45
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+
-
738510
Rhodococcus sp. AD45
N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+
-
-
-
?
2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O
-
738510
Rhodococcus sp. AD45
2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+
-
-
-
?
additional information
no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates
738510
Rhodococcus sp. AD45
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 25000, SDS-PAGE
Rhodococcus sp. AD45
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Rhodococcus sp. AD45
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhodococcus sp. AD45
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhodococcus sp. AD45
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
NAD+
at pH 10.0 and 30°C
Rhodococcus sp. AD45
1.4
-
1-hydroxy-2-glutathionyl-2-methyl-3-butene
at pH 10.0 and 30°C
Rhodococcus sp. AD45
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
2 * 25000, SDS-PAGE
Rhodococcus sp. AD45
Purification (Commentary) (protein specific)
Commentary
Organism
Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration
Rhodococcus sp. AD45
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.9
-
crude extract, at pH 10.0 and 30°C
Rhodococcus sp. AD45
18
-
after 6.2fold purification, at pH 10.0 and 30°C
Rhodococcus sp. AD45
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+
-
738510
Rhodococcus sp. AD45
N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+
-
-
-
?
2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O
-
738510
Rhodococcus sp. AD45
2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+
-
-
-
?
additional information
no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates
738510
Rhodococcus sp. AD45
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 25000, SDS-PAGE
Rhodococcus sp. AD45
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Rhodococcus sp. AD45
Other publictions for EC 1.1.1.398
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738510
Van Hylckama Vlieg
Purification of a glutathione ...
Rhodococcus sp. AD45
J. Bacteriol.
181
2094-2101
1999
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1
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1
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1
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