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Literature summary for 1.1.1.396 extracted from

  • Lange, C.; Kiesel, S.; Peters, S.; Virus, S.; Scheer, H.; Jahn, D.; Moser, J.
    Broadened substrate specificity of 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase (BchC) indicates a new route for the biosynthesis of bacteriochlorophyll a (2015), J. Biol. Chem., 290, 19697-19709.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.396

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlorobaculum tepidum

Protein Variants

Protein Variants Comment Organism
E46A inactive Chlorobaculum tepidum
E68A inactive Chlorobaculum tepidum
H141A inactive Chlorobaculum tepidum
Y67A inactive Chlorobaculum tepidum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Chlorobaculum tepidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+ Chlorobaculum tepidum
-
 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+ Chlorobaculum tepidum TLS
-
 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
3-hydroxyethyl chlorophyllide a + NAD+ Chlorobaculum tepidum
-
 bacteriopheophorbide a + NADH + H+
-
 ?
3-hydroxyethyl chlorophyllide a + NAD+ Chlorobaculum tepidum TLS
-
 bacteriopheophorbide a + NADH + H+
-
 ?
bacteriopheophorbide a + NAD+ Chlorobaculum tepidum
-
 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
bacteriopheophorbide a + NAD+ Chlorobaculum tepidum TLS
-
 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
additional information Chlorobaculum tepidum no activity with 3-hydroxyethyl-bacteriochlorophyll a ?
-
 ?
additional information Chlorobaculum tepidum TLS no activity with 3-hydroxyethyl-bacteriochlorophyll a ?
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KCJ7
-
-
Chlorobaculum tepidum TLS Q8KCJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-agarose column chromatography, and gel filtration Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+
-
 Chlorobaculum tepidum 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+
-
 Chlorobaculum tepidum TLS 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
3-hydroxyethyl chlorophyllide a + NAD+
-
 Chlorobaculum tepidum bacteriopheophorbide a + NADH + H+
-
 ?
3-hydroxyethyl chlorophyllide a + NAD+
-
 Chlorobaculum tepidum TLS bacteriopheophorbide a + NADH + H+
-
 ?
bacteriopheophorbide a + NAD+
-
 Chlorobaculum tepidum 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
bacteriopheophorbide a + NAD+
-
 Chlorobaculum tepidum TLS 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
additional information no activity with 3-hydroxyethyl-bacteriochlorophyll a Chlorobaculum tepidum ?
-
 ?
additional information no activity with 3-hydroxyethyl-bacteriochlorophyll a Chlorobaculum tepidum TLS ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Chlorobaculum tepidum
? x * 37068, calculated from amino acid sequence Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
3-hydroxyethyl bacteriochlorophyllide a dehydrogenase
-
 Chlorobaculum tepidum
bchC
-
 Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Chlorobaculum tepidum