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Literature summary for 1.1.1.392 extracted from

  • Devlin, A.; Fischbach, M.
    A biosynthetic pathway for a prominent class of microbiota-derived bile acids (2015), Nat. Chem. Biol., 11, 685-690.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.392

Cloned(Commentary)

Cloned (Comment) Organism
gene Elen_0690, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli Eggerthella lenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1280
-
 12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid pH 7.0, 37°C Eggerthella lenta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ Eggerthella lenta
-
 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ Eggerthella lenta ATCC 25559
-
 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Eggerthella lenta C8WMP0 gene Elen_0690
-
Eggerthella lenta ATCC 25559 C8WMP0 gene Elen_0690
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 Eggerthella lenta 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 Eggerthella lenta ATCC 25559 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
3alpha-HSDH
-
 Eggerthella lenta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Eggerthella lenta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.85
-
 12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid pH 7.0, 37°C Eggerthella lenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Eggerthella lenta

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Eggerthella lenta

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids Eggerthella lenta
physiological function the iso–bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides Eggerthella lenta