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Literature summary for 1.1.1.391 extracted from

  • Edenharder, R.; Pfuetzner, M.
    Partial purification and characterization of an NAD-dependent 3beta-hydroxysteroid dehydrogenase from Clostridium innocuum (1989), Appl. Environ. Microbiol., 55, 1656-1659.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.391

General Stability

General Stability Organism
EDTA has no effect enzyme thermal stability [Clostridium] innocuum

Inhibitors

Inhibitors Comment Organism Structure
3-oxo bile acids the specific enzyme activity is significantly reduced by growth medium supplementation with 3-oxo bile acids, with trisubstituted acids being more effective than disubstituted ones [Clostridium] innocuum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetis [Clostridium] innocuum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound [Clostridium] innocuum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 gel filtration [Clostridium] innocuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ [Clostridium] innocuum
-
 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
 ?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ [Clostridium] innocuum D1144/3
-
 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
 ?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ [Clostridium] innocuum
-
 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ [Clostridium] innocuum D1144/3
-
 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
[Clostridium] innocuum
-
-
-
[Clostridium] innocuum D1144/3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 6.3fold by anion exchange chromatography and gel filtration [Clostridium] innocuum

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme production is constitutive in the absence of added bile acids [Clostridium] innocuum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
 purified enzyme, pH 10.0-10.2, 37°C [Clostridium] innocuum

Storage Stability

Storage Stability Organism
4°C, crude enzyme extract, pH 8.5, in the presence of dithiothreitol, no loss of activity after 24 h [Clostridium] innocuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ sole pruduct ?
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum D1144/3 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ sole pruduct ?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
 ?
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum D1144/3 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
 ?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
 ?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum D1144/3 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
 ?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum D1144/3 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 [Clostridium] innocuum 3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
 ?
additional information the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value [Clostridium] innocuum ?
-
 ?
additional information the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value [Clostridium] innocuum D1144/3 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE [Clostridium] innocuum

Synonyms

Synonyms Comment Organism
3beta-HSD
-
 [Clostridium] innocuum
NAD-dependent 3beta-hydroxysteroid dehydrogenase
-
 [Clostridium] innocuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at [Clostridium] innocuum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 crude enzyme extract, pH 8.5, inactivation after 30 min [Clostridium] innocuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 10.02
-
 [Clostridium] innocuum

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on [Clostridium] innocuum

Expression

Organism Comment Expression
[Clostridium] innocuum effect of bile acids on the production of 3beta-HSDH activity in Clostridium innocuum strain D1144/3 down