Cloned (Comment) | Organism |
---|---|
gene maeE, is encoded in a cluster consisting of two diverging operons, maePE and maeKR, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Lacticaseibacillus casei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Lacticaseibacillus casei | - |
pyruvate + NADH + H+ + CO2 | - |
? | |
(S)-malate + NAD+ | Lacticaseibacillus casei BL23 and ATCC 334 | - |
pyruvate + NADH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
gene maeE | - |
Lacticaseibacillus casei BL23 and ATCC 334 | - |
gene maeE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Lacticaseibacillus casei | pyruvate + NADH + H+ + CO2 | - |
? | |
(S)-malate + NAD+ | - |
Lacticaseibacillus casei BL23 and ATCC 334 | pyruvate + NADH + H+ + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Lacticaseibacillus casei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lacticaseibacillus casei |
Organism | Comment | Expression |
---|---|---|
Lacticaseibacillus casei | expression of maeE is repressed by glucose | down |
Lacticaseibacillus casei | expression of maeE is induced in the presence of L-malic acid. MaeR binds specifically to a set of direct repeats [5'-TTATT(A/T)AA-3'] in the mae promoter region and activates the expression of the diverging operons maePE and maeKR | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the malic enzyme is involved in the (S)-malate catabolic pathways and the putative gluconeogenic pathways, overview | Lacticaseibacillus casei |
additional information | deletion of either the gene encoding the histidine kinase or the response regulator of the TC system results in the loss of the ability to grow on L-malic acid, thus indicating that the cognate TC system regulates and is essential for the expression of malic enzyme. Expression of maeE is induced in the presence of L-malic acid and repressed by glucose, whereas TC system expression is induced by L-malic acid and is not repressed by glucose | Lacticaseibacillus casei |
physiological function | the malic enzyme pathway enables Lactobacillus casei to grow on L-malic acid., requirement of the MaeKR two-component system for L-malic acid utilization via a malic enzyme pathway, overview | Lacticaseibacillus casei |