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Literature summary for 1.1.1.39 extracted from

  • Landete, J.M.; Garcia-Haro, L.; Blasco, A.; Manzanares, P.; Berbegal, C.; Monedero, V.; Zuniga, M.
    Requirement of the Lactobacillus casei MaeKR two-component system for L-malic acid utilization via a malic enzyme pathway (2010), Appl. Environ. Microbiol., 76, 84-95.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene maeE, is encoded in a cluster consisting of two diverging operons, maePE and maeKR, DNA and amino acid sequence determination and analysis, phylogenetic analysis Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Lacticaseibacillus casei
-
pyruvate + NADH + H+ + CO2
-
?
(S)-malate + NAD+ Lacticaseibacillus casei BL23 and ATCC 334
-
pyruvate + NADH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
gene maeE
-
Lacticaseibacillus casei BL23 and ATCC 334
-
gene maeE
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Lacticaseibacillus casei pyruvate + NADH + H+ + CO2
-
?
(S)-malate + NAD+
-
Lacticaseibacillus casei BL23 and ATCC 334 pyruvate + NADH + H+ + CO2
-
?

Synonyms

Synonyms Comment Organism
malic enzyme
-
Lacticaseibacillus casei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lacticaseibacillus casei

Expression

Organism Comment Expression
Lacticaseibacillus casei expression of maeE is repressed by glucose down
Lacticaseibacillus casei expression of maeE is induced in the presence of L-malic acid. MaeR binds specifically to a set of direct repeats [5'-TTATT(A/T)AA-3'] in the mae promoter region and activates the expression of the diverging operons maePE and maeKR up

General Information

General Information Comment Organism
metabolism the malic enzyme is involved in the (S)-malate catabolic pathways and the putative gluconeogenic pathways, overview Lacticaseibacillus casei
additional information deletion of either the gene encoding the histidine kinase or the response regulator of the TC system results in the loss of the ability to grow on L-malic acid, thus indicating that the cognate TC system regulates and is essential for the expression of malic enzyme. Expression of maeE is induced in the presence of L-malic acid and repressed by glucose, whereas TC system expression is induced by L-malic acid and is not repressed by glucose Lacticaseibacillus casei
physiological function the malic enzyme pathway enables Lactobacillus casei to grow on L-malic acid., requirement of the MaeKR two-component system for L-malic acid utilization via a malic enzyme pathway, overview Lacticaseibacillus casei