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Literature summary for 1.1.1.39 extracted from

  • Pongratz, R.L.; Kibbey, R.G.; Shulman, G.I.; Cline, G.W.
    Cytosolic and mitochondrial malic enzyme isoforms differentially control insulin secretion (2007), J. Biol. Chem., 282, 200-207.
    View publication on PubMed
Search for more literature for 1.1.1.39

Cloned(Commentary)

Cloned (Comment) Organism
isozyme expression analysis, overview Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information siRNA knockdown of malic enzyme isozyme by 50% affects pyruvate carboxylase flux of the pyruvate derived from glutamate metabolism, insulin secretion in response to membrane depolarization using potassium chloride is unaffected by siRNA knockdown of malic enzyme, overview Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion isozyme ME2 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Rattus norvegicus mitochondrial isozyme ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting pyruvate + CO2 + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 832/13 cell
-
 Rattus norvegicus
-
pancreatic beta cell isozyme ME2 Rattus norvegicus
-
pancreatic islet
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 isozyme expression levels, pyruvate cycling and isozyme activity in pancreatic islets, overview Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Rattus norvegicus pyruvate + CO2 + NADH
-
 r
(S)-malate + NAD+ mitochondrial isozyme ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting Rattus norvegicus pyruvate + CO2 + NADH
-
 r

Synonyms

Synonyms Comment Organism
NAD-malic enzyme
-
 Rattus norvegicus
NAD-ME2
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rattus norvegicus
NADH
-
 Rattus norvegicus