Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | - |
Brevundimonas diminuta | |
EDTA | - |
Brevundimonas diminuta | |
HgCl2 | - |
Brevundimonas diminuta | |
hydroquinone | - |
Brevundimonas diminuta | |
NEM | - |
Brevundimonas diminuta | |
oxalate | - |
Brevundimonas diminuta | |
PCMB | - |
Brevundimonas diminuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.97 | - |
(S)-malate | with NAD+ as coenzyme | Brevundimonas diminuta | |
8.34 | - |
(S)-malate | with NADP+ as coenzyme | Brevundimonas diminuta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent metal ion required, NAD+-linked activity shows maximal activity at 5 mM Mg2+ | Brevundimonas diminuta | |
Ni2+ | divalent metal ion required, NADP+-linked activity exhibits a maximum at 5 mM Ni2+ | Brevundimonas diminuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
10 * 64000, SDS-PAGE | Brevundimonas diminuta |
680000 | - |
gel filtration | Brevundimonas diminuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevundimonas diminuta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.74 | - |
NAD+-linked activity | Brevundimonas diminuta |
5.45 | - |
NADP+-linked activity | Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | - |
Brevundimonas diminuta | pyruvate + CO2 + NADH + H+ | - |
? | |
additional information | no decarboxylation of oxaloacetate | Brevundimonas diminuta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 64000, SDS-PAGE | Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Brevundimonas diminuta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 35% of maximal activity, 60°C: about 50% of maximal activity | Brevundimonas diminuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
15 min, stable at or below | Brevundimonas diminuta |
55 | - |
15 min, complete inactivation | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Brevundimonas diminuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | about 40% of maximal activity at pH 6.0 and at pH 8.5 | Brevundimonas diminuta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 25°C, 5 min, stable | Brevundimonas diminuta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Brevundimonas diminuta | |
NADP+ | activity with NADP+ is about twice that measured with NAD+ | Brevundimonas diminuta |