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Literature summary for 1.1.1.39 extracted from

  • Suye, S.; Okada, Y.; Funada, A.; Kawagoe, M.; Inuta, S.
    Purification and properties of malic enzyme from Pseudomonas diminuta IFO-13182 (1992), J. Ferment. Bioeng., 73, 343-347.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Brevundimonas diminuta
EDTA
-
Brevundimonas diminuta
HgCl2
-
Brevundimonas diminuta
hydroquinone
-
Brevundimonas diminuta
NEM
-
Brevundimonas diminuta
oxalate
-
Brevundimonas diminuta
PCMB
-
Brevundimonas diminuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.97
-
(S)-malate with NAD+ as coenzyme Brevundimonas diminuta
8.34
-
(S)-malate with NADP+ as coenzyme Brevundimonas diminuta

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal ion required, NAD+-linked activity shows maximal activity at 5 mM Mg2+ Brevundimonas diminuta
Ni2+ divalent metal ion required, NADP+-linked activity exhibits a maximum at 5 mM Ni2+ Brevundimonas diminuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
10 * 64000, SDS-PAGE Brevundimonas diminuta
680000
-
gel filtration Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevundimonas diminuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.74
-
NAD+-linked activity Brevundimonas diminuta
5.45
-
NADP+-linked activity Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malate + NAD+
-
Brevundimonas diminuta pyruvate + CO2 + NADH + H+
-
?
additional information no decarboxylation of oxaloacetate Brevundimonas diminuta ?
-
?

Subunits

Subunits Comment Organism
decamer 10 * 64000, SDS-PAGE Brevundimonas diminuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Brevundimonas diminuta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 35% of maximal activity, 60°C: about 50% of maximal activity Brevundimonas diminuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
15 min, stable at or below Brevundimonas diminuta
55
-
15 min, complete inactivation Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Brevundimonas diminuta

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 about 40% of maximal activity at pH 6.0 and at pH 8.5 Brevundimonas diminuta

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 25°C, 5 min, stable Brevundimonas diminuta

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Brevundimonas diminuta
NADP+ activity with NADP+ is about twice that measured with NAD+ Brevundimonas diminuta