Activating Compound | Comment | Organism | Structure |
---|---|---|---|
coenzyme A | increases activity | Panicum miliaceum | |
coenzyme A | increases activity | Atriplex spongiosa | |
coenzyme A | increases activity | Amaranthus edulis | |
SO42- | activates | Atriplex spongiosa |
General Stability | Organism |
---|---|
freezing of crude enzyme extract treated on Sephadex G-25 destroys activity | Atriplex spongiosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | above 10 mM | Atriplex spongiosa | |
bicarbonate | - |
Amaranthus edulis | |
bicarbonate | - |
Atriplex spongiosa | |
HCO3- | - |
Atriplex spongiosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
NAD+ | - |
Atriplex spongiosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no activation by Mg2+ | Panicum miliaceum | |
Mg2+ | no activation by Mg2+ | Atriplex spongiosa | |
Mg2+ | no activation by Mg2+ | Amaranthus edulis | |
Mn2+ | absolute requirement for Mn2+, no activation by Mg2+ | Panicum miliaceum | |
Mn2+ | absolute requirement for Mn2+, no activation by Mg2+ | Atriplex spongiosa | |
Mn2+ | absolute requirement for Mn2+, no activation by Mg2+ | Amaranthus edulis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Atriplex spongiosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amaranthus edulis | - |
- |
- |
Atriplex spongiosa | - |
- |
- |
Panicum miliaceum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Atriplex spongiosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | bundle sheath | Panicum miliaceum | - |
leaf | bundle sheath | Atriplex spongiosa | - |
leaf | bundle sheath | Amaranthus edulis | - |
Storage Stability | Organism |
---|---|
crude and partially purified enzyme retains activity for several months when stored as a protein suspension in 75% saturated (NH4)2SO4 solution | Atriplex spongiosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | 15% of the activity with NAD+ | Atriplex spongiosa | pyruvate + CO2 + NADPH | - |
? | |
malate + NAD+ | - |
Panicum miliaceum | pyruvate + CO2 + NADH + H+ | - |
? | |
malate + NAD+ | - |
Atriplex spongiosa | pyruvate + CO2 + NADH + H+ | - |
? | |
malate + NAD+ | - |
Amaranthus edulis | pyruvate + CO2 + NADH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
with NAD+, without activator | Atriplex spongiosa |
6.9 | - |
activity with NADP+ | Atriplex spongiosa |
7.2 | - |
with NAD+ plus CoA | Atriplex spongiosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Panicum miliaceum | |
NAD+ | cofactor | Atriplex spongiosa | |
NAD+ | cofactor | Amaranthus edulis | |
NADP+ | 15% of the activity with NAD+ | Atriplex spongiosa |