BRENDA - Enzyme Database show
show all sequences of 1.1.1.384

In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa

Hong, L.; Zhao, Z.; Melancon III, C.; Zhang, H.; Liu, H.; J. Am. Chem. Soc. 130, 4954-4967 (2008)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37732
-
1 * 38000, SDS-PAGE, 1 * 37732, calculated, His-tagged recombinant protein
Saccharopolyspora spinosa
38000
-
gel filtration
Saccharopolyspora spinosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
Saccharopolyspora spinosa
-
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharopolyspora spinosa
Q9ALN5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
-
731977
Saccharopolyspora spinosa
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE, 1 * 37732, calculated, His-tagged recombinant protein
Saccharopolyspora spinosa
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH is preferred over NADH
Saccharopolyspora spinosa
NADPH
preferred over NADH
Saccharopolyspora spinosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH is preferred over NADH
Saccharopolyspora spinosa
NADPH
preferred over NADH
Saccharopolyspora spinosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37732
-
1 * 38000, SDS-PAGE, 1 * 37732, calculated, His-tagged recombinant protein
Saccharopolyspora spinosa
38000
-
gel filtration
Saccharopolyspora spinosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
Saccharopolyspora spinosa
-
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
TDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+
-
731977
Saccharopolyspora spinosa
TDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE, 1 * 37732, calculated, His-tagged recombinant protein
Saccharopolyspora spinosa
General Information
General Information
Commentary
Organism
physiological function
enzyme is involved in insecticide spinosyn synthesis. It acts sequentially with 2,3-dehydratase SpnO to catalyze C-2 deoxygenation of TDP-4-dehydro-6-deoxy-D-glucose to form the SpnQ substrate, TDP-4-dehydro-2,6-dideoxy-D-glucose
Saccharopolyspora spinosa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme is involved in insecticide spinosyn synthesis. It acts sequentially with 2,3-dehydratase SpnO to catalyze C-2 deoxygenation of TDP-4-dehydro-6-deoxy-D-glucose to form the SpnQ substrate, TDP-4-dehydro-2,6-dideoxy-D-glucose
Saccharopolyspora spinosa
Other publictions for EC 1.1.1.384
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729208
Kubiak
Combined structural and functi ...
Actinomadura kijaniata
Biochemistry
50
5905-5917
2011
-
-
1
1
4
-
-
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
5
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1
731977
Hong
In vitro characterization of t ...
Saccharopolyspora spinosa
J. Am. Chem. Soc.
130
4954-4967
2008
-
-
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732465
Wang
Biosynthesis of the dideoxysug ...
Streptomyces venezuelae
Microbiology
148
1091-1103
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731140
Aguirrezabalaga
Identification and expression ...
Streptomyces antibioticus
Antimicrob. Agents Chemother.
44
1266-1275
2000
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-