Literature summary for 1.1.1.382 extracted from

Cahn, J.K.; Brinkmann-Chen, S.; Spatzal, T.; Wiig, J.A.; Buller, A.R.; Einsle, O.; Hu, Y.; Ribbe, M.W.; Arnold, F.H.
Cofactor specificity motifs and the induced fit mechanism in Class I ketol-acid reductoisomerases (2015), Biochem. J., 468, 475-484.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
apo-enzyme, to 1.39 A resolution, and in complex with NADP+. Enzyme accepts both cofactors NADH and NADPH. Insertions near the N-terminus of the specificity loop probably determine cofactor specificity, the laboratory engineering of NADH preference involved specific loop substitution	Ignisphaera aggregans
in complex with NADH, to 1.54 A resolution. The specificity loop differs significantly from any other crystallized ketol-acid reductoisomerases. Glu46, the first residue of the beta2alphaB-loop, lies along the N3 edge of the adenine moiety and forms a bi-dentate interaction with the O2' hydroxy of the ribose. The position of this glutamate is stabilized by a hydrogen bond interaction with Asn55	uncultured archaeon
in complex with substrate analog L-tartaric acid, to 2.5 A resolution. The enzyme displays a six-member specificity loop, with residues Arg48 and Ser52 forming hydrogen bonds to the NAD(H) phosphate	Alicyclobacillus acidocaldarius subsp. acidocaldarius
to 1.54 A resolution. Structure contains 2 Mg2+ ions and NADH. Glu46, the first residue of the beta2alphaB-loop, lies along the N3 edge of the adenine moiety and forms a bi-dentate interaction with the O2 hydroxy of the ribose. The position of this glutamate is stabilized by a hydrogen bond interaction with Asn55	uncultured archaeon GZfos26G2

Organism

Organism	UniProt	Comment	Textmining
Alicyclobacillus acidocaldarius subsp. acidocaldarius	C8WR67	cf. EC 1.1.1.86	-
Ignisphaera aggregans	E0SRA9	-	-
Ignisphaera aggregans DSM 17230	E0SRA9	-	-
uncultured archaeon	Q64BR7	cf. 1.1.1.86	-
uncultured archaeon GZfos26G2	Q64BR7	-	-

Synonyms

Synonyms	Comment	Organism
ilvC	-	uncultured archaeon GZfos26G2
ilvC	-	Ignisphaera aggregans
ilvC	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius
ilvC	-	uncultured archaeon

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Release 2023.1 (January 2023)