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Literature summary for 1.1.1.38 extracted from
- Structural insights into malic enzyme variants favoring a non-natural redox cofactor (2021), ChemBioChem, 22, 1765-1768 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L310R | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/Q401C | the mutant prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/R346A/Q401C | the mutant prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/R346D/Q401C | the mutant prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/R346F/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/R346K/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
| L310R/R346Y/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
NAD+ | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli |  |
| 0.13 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.27 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 0.29 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.44 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.76 | - |
NAD+ | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 1.11 | - |
NAD+ | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 2.31 | - |
NAD+ | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 4.83 | - |
NAD+ | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 5 | - |
NAD+ | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
| 5 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 5.32 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 9.4 | - |
nicotinamide cytosine dinucleotide | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 10.24 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 17.2 | - |
NAD+ | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 102 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Escherichia coli | - |
pyruvate + CO2 + NADH | - |
r | |
| pyruvate + CO2 + NADH | Escherichia coli | - |
(S)-malate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P26616 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Escherichia coli | pyruvate + CO2 + NADH | - |
r | |
| (S)-malate + NAD+ | - |
Escherichia coli | pyruvate + CO2 + NADH + H+ | - |
r | |
| (S)-malate + nicotinamide cytosine dinucleotide | - |
Escherichia coli | ? | - |
r | |
| pyruvate + CO2 + NADH | - |
Escherichia coli | (S)-malate + NAD+ | - |
r | |
| pyruvate + CO2 + NADH + H+ | - |
Escherichia coli | (S)-malate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malic enzyme | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
NAD+ | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.02 | - |
NAD+ | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.08 | - |
NAD+ | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.08 | - |
NAD+ | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.12 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.18 | - |
NAD+ | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.6 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
| 0.64 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.86 | - |
NAD+ | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 1.53 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 2.69 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 3.38 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 5.6 | - |
NAD+ | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
| 9.33 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 26.1 | - |
nicotinamide cytosine dinucleotide | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 57.3 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| nicotinamide cytosine dinucleotide | - |
Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.02 | - |
NAD+ | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.03 | - |
NAD+ | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.05 | - |
NAD+ | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.07 | - |
NAD+ | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.08 | - |
NAD+ | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.22 | - |
NAD+ | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.31 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 0.9 | - |
NAD+ | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
| 0.91 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 2.21 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 2.8 | - |
nicotinamide cytosine dinucleotide | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 7.68 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 20.69 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
| 169.9 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
| 214.8 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
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Release 2023.1 (January 2023)
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