Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in strain BL21(DE3) as mainly soluble protein | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
NAD+ | pH 7.2, 25°C, recombinant enzyme | Escherichia coli K-12 | |
0.42 | - |
(S)-malate | pH 7.2, 25°C, recombinant enzyme | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
monomeric recombinant His6-tagged enzyme, native PAGE | Escherichia coli K-12 |
260000 | - |
tetrameric recombinant His6-tagged enzyme, native PAGE | Escherichia coli K-12 |
500000 | - |
octameric recombinant His6-tagged enzyme, native PAGE | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Escherichia coli K-12 | via decarboxylation of oxaloacetate | pyruvate + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to 95% purity | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | via decarboxylation of oxaloacetate | Escherichia coli K-12 | pyruvate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 65000, recombinant His6-tagged enzyme, SDS-PAGE, monomer, homotetramer, and homooctamer enzyme fomrs exist in solution | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent malic enzyme | - |
Escherichia coli K-12 |
NAD-malic enzyme | - |
Escherichia coli K-12 |
NAD-ME | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
134.4 | - |
(S)-malate | pH 7.2, 25°C, recombinant enzyme | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
recombinant His6-tagged enzyme | Escherichia coli K-12 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 8 | over 70% of maximal activity within this range | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Escherichia coli K-12 |