BRENDA - Enzyme Database
show all sequences of 1.1.1.377

Characterization of NADP+-specific L-rhamnose dehydrogenase from the thermoacidophilic Archaeon Thermoplasma acidophilum.

Kim, S.M.; Paek, K.H.; Lee, S.B.; Extremophiles 16, 447-454 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Thermoplasma acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADP+
pH 7.0, 60°C, cosubstrate: L-lyxose
Thermoplasma acidophilum
0.1
-
NADP+
pH 7.0, 60°C, cosubstrate: L-rhamnose
Thermoplasma acidophilum
0.13
-
NADP+
pH 7.0, 60°C, cosubstrate: L-mannose
Thermoplasma acidophilum
0.46
-
L-rhamnose
pH 7.0, 60°C
Thermoplasma acidophilum
1.37
-
L-Lyxose
pH 7.0, 60°C
Thermoplasma acidophilum
6.74
-
L-Mannose
pH 7.0, 60°C
Thermoplasma acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31237
-
x * 31237, calculated from sequence4
Thermoplasma acidophilum
34000
-
x * 34000, SDS-PAGE
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-rhamnose + NADP+
Thermoplasma acidophilum
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
L-rhamno-1,4-lactone + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermoplasma acidophilum
G3LGQ3
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.2
-
pH 7.0, 60°C, substrates: L-mannose + NADP+
Thermoplasma acidophilum
35.1
-
pH 7.0, 60°C, substrates: L-lyxose + NADP+
Thermoplasma acidophilum
36.2
-
pH 7.0, 60°C, substrates: L-rhamnose + NADP+
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-lyxose + NADP+
-
727435
Thermoplasma acidophilum
?
-
-
-
?
L-mannose + NADP+
-
727435
Thermoplasma acidophilum
?
-
-
-
?
L-rhamnose + NADP+
-
727435
Thermoplasma acidophilum
L-rhamno-1,4-lactone + NADPH + H+
-
-
-
?
L-rhamnose + NADP+
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
727435
Thermoplasma acidophilum
L-rhamno-1,4-lactone + NADPH + H+
-
-
-
?
additional information
less than 1% activity is observed for D-mannose, myo-inositol, D-glyceraldehyde, and D-talose. No catalytic activity is detected for the following substrates: D-glucose, D-galactose, L-galactose, D-arabinose, L-arabinose, Dxylose, L-xylose, D-fucose, L-fucose, D-fructose, D-mannitol, D-xylitol, D-sorbitol, D-ribose, D-deoxyribose, D-glucosamine, N-acetyl-D-glucosamine, and D-altrose
727435
Thermoplasma acidophilum
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34000, SDS-PAGE
Thermoplasma acidophilum
?
x * 31237, calculated from sequence4
Thermoplasma acidophilum
Synonyms
Synonyms
Commentary
Organism
NADP+-specific L-rhamnose dehydrogenase
-
Thermoplasma acidophilum
RhaD
-
Thermoplasma acidophilum
Ta0747P
locus name
Thermoplasma acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
activity towards L-rhamnose
Thermoplasma acidophilum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14.88
-
L-Mannose
pH 7.0, 60°C
Thermoplasma acidophilum
16.5
-
NADP+
pH 7.0, 60°C, cosubstrate: L-lyxose
Thermoplasma acidophilum
17.1
-
NADP+
pH 7.0, 60°C, cosubstrate: L-rhamnose
Thermoplasma acidophilum
18.08
-
NADP+
pH 7.0, 60°C, cosubstrate: L-mannose
Thermoplasma acidophilum
22.36
-
L-rhamnose
pH 7.0, 60°C
Thermoplasma acidophilum
23.77
-
L-Lyxose
pH 7.0, 60°C
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
activity towards L-rhamnose
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Thermoplasma acidophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Thermoplasma acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Thermoplasma acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADP+
pH 7.0, 60°C, cosubstrate: L-lyxose
Thermoplasma acidophilum
0.1
-
NADP+
pH 7.0, 60°C, cosubstrate: L-rhamnose
Thermoplasma acidophilum
0.13
-
NADP+
pH 7.0, 60°C, cosubstrate: L-mannose
Thermoplasma acidophilum
0.46
-
L-rhamnose
pH 7.0, 60°C
Thermoplasma acidophilum
1.37
-
L-Lyxose
pH 7.0, 60°C
Thermoplasma acidophilum
6.74
-
L-Mannose
pH 7.0, 60°C
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31237
-
x * 31237, calculated from sequence4
Thermoplasma acidophilum
34000
-
x * 34000, SDS-PAGE
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-rhamnose + NADP+
Thermoplasma acidophilum
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
L-rhamno-1,4-lactone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.2
-
pH 7.0, 60°C, substrates: L-mannose + NADP+
Thermoplasma acidophilum
35.1
-
pH 7.0, 60°C, substrates: L-lyxose + NADP+
Thermoplasma acidophilum
36.2
-
pH 7.0, 60°C, substrates: L-rhamnose + NADP+
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-lyxose + NADP+
-
727435
Thermoplasma acidophilum
?
-
-
-
?
L-mannose + NADP+
-
727435
Thermoplasma acidophilum
?
-
-
-
?
L-rhamnose + NADP+
-
727435
Thermoplasma acidophilum
L-rhamno-1,4-lactone + NADPH + H+
-
-
-
?
L-rhamnose + NADP+
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
727435
Thermoplasma acidophilum
L-rhamno-1,4-lactone + NADPH + H+
-
-
-
?
additional information
less than 1% activity is observed for D-mannose, myo-inositol, D-glyceraldehyde, and D-talose. No catalytic activity is detected for the following substrates: D-glucose, D-galactose, L-galactose, D-arabinose, L-arabinose, Dxylose, L-xylose, D-fucose, L-fucose, D-fructose, D-mannitol, D-xylitol, D-sorbitol, D-ribose, D-deoxyribose, D-glucosamine, N-acetyl-D-glucosamine, and D-altrose
727435
Thermoplasma acidophilum
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34000, SDS-PAGE
Thermoplasma acidophilum
?
x * 31237, calculated from sequence4
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
activity towards L-rhamnose
Thermoplasma acidophilum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14.88
-
L-Mannose
pH 7.0, 60°C
Thermoplasma acidophilum
16.5
-
NADP+
pH 7.0, 60°C, cosubstrate: L-lyxose
Thermoplasma acidophilum
17.1
-
NADP+
pH 7.0, 60°C, cosubstrate: L-rhamnose
Thermoplasma acidophilum
18.08
-
NADP+
pH 7.0, 60°C, cosubstrate: L-mannose
Thermoplasma acidophilum
22.36
-
L-rhamnose
pH 7.0, 60°C
Thermoplasma acidophilum
23.77
-
L-Lyxose
pH 7.0, 60°C
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
activity towards L-rhamnose
Thermoplasma acidophilum
General Information
General Information
Commentary
Organism
physiological function
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
Thermoplasma acidophilum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
Thermoplasma acidophilum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.2
-
L-Mannose
pH 7.0, 60°C
Thermoplasma acidophilum
17.35
-
L-Lyxose
pH 7.0, 60°C
Thermoplasma acidophilum
48.6
-
L-rhamnose
pH 7.0, 60°C
Thermoplasma acidophilum
139
-
NADP+
pH 7.0, 60°C, cosubstrate: L-mannose
Thermoplasma acidophilum
165
-
NADP+
pH 7.0, 60°C, cosubstrate: L-lyxose
Thermoplasma acidophilum
171
-
NADP+
pH 7.0, 60°C, cosubstrate: L-rhamnose
Thermoplasma acidophilum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.2
-
L-Mannose
pH 7.0, 60°C
Thermoplasma acidophilum
17.35
-
L-Lyxose
pH 7.0, 60°C
Thermoplasma acidophilum
48.6
-
L-rhamnose
pH 7.0, 60°C
Thermoplasma acidophilum
139
-
NADP+
pH 7.0, 60°C, cosubstrate: L-mannose
Thermoplasma acidophilum
165
-
NADP+
pH 7.0, 60°C, cosubstrate: L-lyxose
Thermoplasma acidophilum
171
-
NADP+
pH 7.0, 60°C, cosubstrate: L-rhamnose
Thermoplasma acidophilum
Other publictions for EC 1.1.1.377
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727435
Kim
Characterization of NADP+-spec ...
Thermoplasma acidophilum
Extremophiles
16
447-454
2012
-
-
1
-
-
-
-
6
-
-
2
1
-
11
-
-
1
-
-
-
3
-
5
2
3
1
-
-
6
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
6
-
-
2
1
-
-
-
1
-
-
3
-
5
2
1
-
-
6
1
-
-
-
-
1
1
-
6
6