Cloned (Comment) | Organism |
---|---|
gene YlMDH1 | Yarrowia lipolytica |
gene YlMDH2, subject for alternative splicing, DNA and amino acid sequence determination and analysis, phylogenetic analysis, construction and generation of three different splicing variants of YlMDH2 and transcriptional regulation analysis, overview | Yarrowia lipolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | gene YlMDH2 encodes the cytosolic and peroxisomal forms of MDH | Yarrowia lipolytica | 5829 | - |
mitochondrion | - |
Yarrowia lipolytica | 5739 | - |
peroxisome | gene YlMDH2 encodes the cytosolic and peroxisomal forms of MDH | Yarrowia lipolytica | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Yarrowia lipolytica | - |
oxaloacetate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | Q6C5X9 | gene YlMDH2; several strains, overview, gene YALI0E14190g or YlMDH2 | - |
Yarrowia lipolytica | Q6C8V3 | isozyme YlMDH1; gene YALI0D16753g or YlMDH1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Yarrowia lipolytica | oxaloacetate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YlMdh2p | - |
Yarrowia lipolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Yarrowia lipolytica |
General Information | Comment | Organism |
---|---|---|
evolution | the three different enzyme forms in cytosol, peroxisome and mitochondrion are encoded by three different genes in Saccharomyces cerevisiae, but by only two genes in Yarrowia lipolytica, where the second gene is differentiated into cytosolic and peroxisomal isozymes by alternative splicing, overview | Yarrowia lipolytica |
malfunction | absence of either the peroxisomal or the cytosolic form of the MDH does not affect growth rate, irrespective of the carbon source | Yarrowia lipolytica |
additional information | possible role for alternative splicing in the regulation of MDH compartmentalization in this yeast, gene YlMDH2 encodes the cytosolic and peroxisomal forms of MDH | Yarrowia lipolytica |