Cloned (Comment) | Organism |
---|---|
gene mdh, expression of wild-type and mutant enzymes in strains DH10B and W945T1-2 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F144I | site-directed mutagenesis, inactive mutant | Escherichia coli |
G210A | site-directed mutagenesis, the mutant shows 30% reduced activity compared to the wild-type enzyme | Escherichia coli |
G210A/V214I | site-directed mutagenesis, the double mutant shows a 2.2fold increase in lacatate dehydrogenase activity compared to the wild-type enzyme | Escherichia coli |
I12V/R81Q/M85E/G210A/V214I | construction of a pentamutant by site-directed mutagenesis, whose substrate specificity is switched from malate dehydrogenase to that of lactate dehydrogenase, EC 1.1.1.27, the mutant shows highly reduced activity compared to the wild-type enzyme, overview | Escherichia coli |
N122D | site-directed mutagenesis, inactive mutant | Escherichia coli |
R81Q | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Escherichia coli |
V214I | site-directed mutagenesis, the mutant's activity is not affected | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
oxaloacetate | pH 7.5, 30°C,recombinant wild-type enzyme | Escherichia coli | |
3 | - |
oxaloacetate | pH 7.5, 30°C, recombinant mutant R81Q | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Escherichia coli | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Escherichia coli MG1655 | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P61889 | gene mdh | - |
Escherichia coli MG1655 | P61889 | gene mdh | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity | Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Escherichia coli MG1655 | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity | Escherichia coli MG1655 | oxaloacetate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malate dehydrogenase | - |
Escherichia coli |
MDH | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
oxaloacetate | pH 7.5, 30°C, recombinant mutant R81Q | Escherichia coli | |
931 | - |
oxaloacetate | pH 7.5, 30°C, recombinant wild-type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |