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Literature summary for 1.1.1.37 extracted from

  • Lin, J.J.; Yang, T.H.; Wahlstrand, B.D.; Fields, P.A.; Somero, G.N.
    Phylogenetic relationships and biochemical properties of the duplicated cytosolic and mitochondrial isoforms of malate dehydrogenase from a teleost fish,Sphyraena idiastes (2002), J. Mol. Evol., 54, 107-117.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphyraena idiastes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
 oxaloacetate pH 7.5, 20°C, cytosolic isoenzyme cMDH Sus scrofa
0.0113
-
 oxaloacetate pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-L Sphyraena idiastes
0.0113
-
 oxaloacetate isoform cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes
0.0134
-
 NADH pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-L Sphyraena idiastes
0.0134
-
 NADH isoform r-cMDH-L, at pH 7.5 and 20°C Sphyraena idiastes
0.0135
-
 oxaloacetate pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sphyraena idiastes
0.0135
-
 oxaloacetate isoform r-cMDH-L, at pH 7.5 and 20°C Sphyraena idiastes
0.0145
-
 NADH pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sus scrofa
0.0205
-
 NADH pH 7.5, 20°C, cytosolic isoenzyme cMDH Sus scrofa
0.0207
-
 NADH pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-S Sphyraena idiastes
0.0207
-
 NADH isoform r-cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes
0.0214
-
 NADH pH 7.5, 20°C, cytosolic isoenzyme cMDH-S Sphyraena idiastes
0.0214
-
 NADH isoform cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes
0.0244
-
 NADH pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sphyraena idiastes
0.0244
-
 NADH isoform r-mMDH, at pH 7.5 and 20°C Sphyraena idiastes
0.0302
-
 oxaloacetate pH 7.5, 20°C, cytosolic isoenzyme cMDH-S Sphyraena idiastes
0.0302
-
 oxaloacetate isoform r-mMDH, at pH 7.5 and 20°C Sphyraena idiastes
0.0348
-
 oxaloacetate pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sus scrofa
0.039
-
 oxaloacetate pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-S Sphyraena idiastes
0.039
-
 oxaloacetate isoform r-cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sphyraena idiastes 5829
-
mitochondrion
-
 Sphyraena idiastes 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Sphyraena idiastes mitochondrial enzyme form mMDH functions in citric acid cycle and in the malate-aspartate shuttle oxaloacetate + NADH + H+
-
 ?
oxaloacetate + NADH Sphyraena idiastes
-
 L-malate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Sphyraena idiastes Q90YZ7 isoenzyme mMDH; Pelican barracuda
-
Sphyraena idiastes Q90YZ8 isoenzyme cMDH-L; Pelican barracuda
-
Sphyraena idiastes Q90YZ9 isoenzyme cMDH-S; Pelican barracuda
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphyraena idiastes

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle white Sphyraena idiastes
-
white muscle
-
 Sphyraena idiastes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ mitochondrial enzyme form mMDH functions in citric acid cycle and in the malate-aspartate shuttle Sphyraena idiastes oxaloacetate + NADH + H+
-
 ?
oxaloacetate + NADH
-
 Sus scrofa L-malate + NAD+
-
 ?
oxaloacetate + NADH
-
 Sphyraena idiastes L-malate + NAD+
-
 ?
oxaloacetate + NADH + H+
-
 Sphyraena idiastes (S)-malate + NAD+
-
 r

Synonyms

Synonyms Comment Organism
cMDH cytosolic isoenzyme Sus scrofa
cMDH -S
-
 Sphyraena idiastes
cMDH-L
-
 Sphyraena idiastes
mMDH mitochondrial isoenzyme Sus scrofa
mMDH mitochondrial isoenzyme Sphyraena idiastes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 about 20% loss of activity of cytosolic enzyme form cMDH after 50 h, about 70% loss of activity of mitochondrial enzyme form mMDH after 10 min Sus scrofa
42
-
 half-life of mMDH is about 5 min Sphyraena idiastes
42
-
 recombinant cytosolic isoenzyme c-MDH-S has a half-life of more than 500 min Sphyraena idiastes
42
-
 recombinant cytosolic isoenzyme form cMDH-L has a half-life of 3 min Sphyraena idiastes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sphyraena idiastes
NADH
-
 Sus scrofa
NADH
-
 Sphyraena idiastes