Crystallization (Comment) | Organism |
---|---|
hanging drop vapour-diffusion method | Chloroflexus aurantiacus |
Protein Variants | Comment | Organism |
---|---|---|
E165K | increase in temperature-optimum to 65°C, compared to 60°C for the wild-type enzyme. Maximal specific activity at temperature optimum is increased by about 30% compared to wild-type enzyme. Melting temperature at pH 4.4 is by 4.6°C, melting temperature at pH 6.0 is increased by 12.0°C and melting temperature at pH 7.5 is increased by 23.9°C compared to wild-type enzyme, mutation stabilizes to such an extent that disruption of the inter-dimer electrostatic network around residue 165 no longer limits kinetic thermal stability | Chloroflexus aurantiacus |
E165Q | increase in temperature-optimum to 65°C, compared to 60°C for the wild-type enzyme. Maximal specific activity at temperature optimum is increased by about 30% compared to wild-type enzyme. Melting temperature at pH 4.4 is by 5.4°C, melting temperature at pH 6.0 is increased by 11.2°C and melting temperature at pH 7.5 is increased by 23.6°C compared to wild-type enzyme, mutation stabilizes to such an extent that disruption of the inter-dimer electrostatic network around residue 165 no longer limits kinetic thermal stability | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Chloroflexus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | P80040 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH | - |
Chloroflexus aurantiacus | L-malate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Chloroflexus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
CaMDH | - |
Chloroflexus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild-type enzyme | Chloroflexus aurantiacus |
65 | - |
mutant enzyme E165Q and E165K | Chloroflexus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | 55°C: about 70% of maximal activity, 65°C: about 10% of maximal activity, wild-type enzyme | Chloroflexus aurantiacus |
55 | 70 | 55°C: about 45% of maximal activity, 70°C: about 75% of maximal activity, mutant enzyme E165Q and E165K | Chloroflexus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
melting temperature of wild-type enzyme at pH 7.5 is 67.8°C | Chloroflexus aurantiacus |
75 | - |
half-life at pH 7.5: 0.6 min for wild-type enzyme, 228 min for mutant enzyme E165Q, 338 min for mutant enzyme E165K | Chloroflexus aurantiacus |
79 | - |
melting temperature of wild-type enzyme at pH 6.0 is 79.4°C | Chloroflexus aurantiacus |
80 | - |
melting temperature of wild-type enzyme at pH 4.4 is 80.4°C | Chloroflexus aurantiacus |
85 | - |
half-life at pH 7.5: 35 min for mutant enzyme E165Q, 46 min for mutant enzyme E165K | Chloroflexus aurantiacus |
85 | - |
melting temperature of mutant enzyme E165K at pH 4.4 | Chloroflexus aurantiacus |
86 | - |
melting temperature of mutant enzyme E165Q at pH 4.4 is 85.8°C | Chloroflexus aurantiacus |
91 | - |
melting temperature of mutant enzyme E165Q at pH 6.0 is 90.6°C, melting temperature of mutant enzyme E165Q at pH 7.5 or mutant enzyme E165K at pH 6.0 is 91.4°C | Chloroflexus aurantiacus |
92 | - |
melting temperature of mutant enzyme E165K at pH 7.5 is 91.7°C | Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Chloroflexus aurantiacus | |
NADH | - |
Chloroflexus aurantiacus |