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Literature summary for 1.1.1.37 extracted from

  • Genda, T.; Nakamatsu, T.; Ozaki, H.
    Purification and characterization of malate dehydrogenase from Corynebacterium glutamicum (2003), J. Biosci. Bioeng., 95, 562-566.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
citrate oxidation of malate to oxaloacetate Corynebacterium glutamicum
isocitrate oxidation of malate to oxaloacetate Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 reduction of oxaloacetate to malate and oxidation of malate to oxaloacetate Corynebacterium glutamicum
2-oxoglutarate reduction of oxaloacetate to malate and oxidation of malate to oxaloacetate Corynebacterium glutamicum
citrate reduction of oxaloacetate to malate Corynebacterium glutamicum
isocitrate reduction of oxaloacetate to malate Corynebacterium glutamicum
succinate slight inhibition of reduction of oxaloacetate Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
oxaloacetate pH 6.5, 20-23°C Corynebacterium glutamicum
0.041
-
NADH pH 8.0, 20-23°C Corynebacterium glutamicum
0.057
-
oxaloacetate pH 8.0, 20-23°C Corynebacterium glutamicum
0.07
-
NADH pH 6.5, 20-23°C Corynebacterium glutamicum
0.1
-
NADH pH 9.5, 20-23°C Corynebacterium glutamicum
0.2
-
oxaloacetate pH 9.5, 20-23°C Corynebacterium glutamicum
0.25
-
NADPH pH 6.5, 20-23°C Corynebacterium glutamicum
0.4
-
NAD+ pH 10.5, 20-23°C Corynebacterium glutamicum
0.55
-
oxaloacetate pH 6.5, 20.23°C Corynebacterium glutamicum
0.8
-
L-malate pH 10.5, 20-23°C Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, SDS-PAGE Corynebacterium glutamicum
130000
-
gel filtration Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NN33 ATCC 14067
-

Purification (Commentary)

Purification (Comment) Organism
-
Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
517
-
-
Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Corynebacterium glutamicum oxaloacetate + NADH + H+
-
r
(S)-malate + NADP+
-
Corynebacterium glutamicum oxaloacetate + NADPH + H+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reduction of oxaloacetate to L-malate with NADH as coenzyme, at oxaloacetate concentration of 0.05 mM. Reduction of oxalacetate to L-malate with NADPH as coenzyme Corynebacterium glutamicum
8
-
reduction of oxaloacetate to L-malate with NADH as coenzyme, at oxaloacetate concentration of 0.1 mM Corynebacterium glutamicum
9
-
around, activity is to low to depict a pH curve Corynebacterium glutamicum
9.5
-
reduction of oxaloacetate to L-malate with NADH as coenzyme, at oxaloacetate concentration of 1.0 mM Corynebacterium glutamicum
10.5
-
oxidation of L-malate to oxalacetete with NAD+ as coenzyme Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Corynebacterium glutamicum
NADH almost equally active both for NADH and NADPH on the bases of the turnover number values at pH 6.5, which is optimal for both coenzymes Corynebacterium glutamicum
NADP+
-
Corynebacterium glutamicum
NADPH almost equally active both for NADH and NADPH on the bases of the turnover number values at pH 6.5, which is optimal for both coenzymes Corynebacterium glutamicum