Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
expression in Escherichia coli | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43600 | - |
x * 43600, SDS-PAGE | Pseudomonas aeruginosa |
43600 | - |
x * 43600, SDS-PAGE | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa O11 | - |
- |
- |
Staphylococcus aureus | P95700 | - |
- |
Staphylococcus aureus Newman | P95700 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, 40% glycerol, stable for several weeks | Pseudomonas aeruginosa |
-20°C, 40% glycerol, stable for several weeks | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+ | - |
Staphylococcus aureus | UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ | enzyme Cap5F is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose | ? | |
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+ | - |
Pseudomonas aeruginosa | UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ | enzyme WbjC is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose | ? | |
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+ | - |
Staphylococcus aureus Newman | UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ | enzyme Cap5F is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose | ? | |
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+ | - |
Pseudomonas aeruginosa O11 | UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ | enzyme WbjC is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43600, SDS-PAGE | Pseudomonas aeruginosa |
? | x * 43600, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
Cap5F | - |
Staphylococcus aureus |
O11 O-antigen biosynthesis associated epimerase | - |
Pseudomonas aeruginosa |
WbjC | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | involved in biosynthesis of N-acetyl-L-fucosamine. Enzyme Cap5E catalyzes 4,6-dehydration of UDP-N-acetyl-Dglucosamine and 3- and 5-epimerization to yield a mixture of three keto-deoxy-sugars. UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose is subsequently reduced at C4 to UDP-2-acetamido-2,6-dideoxy-L-talose by Cap5F. Incubation of UDP-2-acetamido-2,6-dideoxy-L-talose with Cap5G results in 2-epimerization to N-acetyl-L-fucosamine | Staphylococcus aureus |
physiological function | involved in biosynthesis of N-acetyl-L-fucosamine. Enzyme WbjB catalyzes 4,6-dehydration of UDP-N-acetyl-Dglucosamine and 3- and 5-epimerization to yield a mixture of three keto-deoxy-sugars. UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose is subsequently reduced at C4 to UDP-2-acetamido-2,6-dideoxy-L-talose by WbjC. Incubation of UDP-2-acetamido-2,6-dideoxy-L-talose with WbjD results in 2-epimerization to N-acetyl-L-fucosamine | Pseudomonas aeruginosa |