BRENDA - Enzyme Database
show all sequences of 1.1.1.365

Ectopic expression of FaGalUR leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato

Cai, X.; Zhang, C.; Ye, J.; Hu, T.; Ye, Z.; Li, H.; Zhang, Y.; Plant Growth Regul. 76, 187-197 (2015)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
accumulation of high levels of ascorbate using GalUR gene overepression in tomato fruits. Tomato fruits are considered a major dietary source of vitamin C in many countries, because it is consumed regularly and in large quantities. Tomato also serves as a fruit model for other crops species with fleshy berry. Accordingly, it is very important to monitor and to increase the vitamin C content in tomato fruit, for meeting the consumer demand and health requirements for high nutrition, e.g. by overexpressing D-galacturonate reductase
Fragaria x ananassa
Cloned(Commentary)
Commentary
Organism
gene GalUR, recombinant overexpression in Solanum lycopersicum cv. Ailsa Craig leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection resulting in 2fold and 1.6fold increase in ascorbate level in tomato fruit and leaf, respectively, which correlates positively with FaGalUR transcriptional abundance and enzyme GalUR activity compared to wild-type plants, real-time PCR expression analysis
Fragaria x ananassa
Engineering
Amino acid exchange
Commentary
Organism
additional information
gene FaGalUR-overexpressing Solanum lycopersicum plants show enhanced ascorbic acid levels, tolerance to abiotic stresses induced by oxidization (methyl viologen), salt (NaCl), and cold compared to the wild-type plants, overview. Ascorbate accumulation in tomato can be enhanced by regulating Fragaria x ananassa GalUR gene
Fragaria x ananassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Fragaria x ananassa
-
L-galactonate + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fragaria x ananassa
O49133
cv. Fenglaixiang, Fragaria chiloensis x Fragaria virginiana
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Fragaria x ananassa
-
leaf
-
Fragaria x ananassa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
743465
Fragaria x ananassa
L-galactonate + NADP+
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Fragaria x ananassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Fragaria x ananassa
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Fragaria x ananassa
NADPH
-
Fragaria x ananassa
Application (protein specific)
Application
Commentary
Organism
nutrition
accumulation of high levels of ascorbate using GalUR gene overepression in tomato fruits. Tomato fruits are considered a major dietary source of vitamin C in many countries, because it is consumed regularly and in large quantities. Tomato also serves as a fruit model for other crops species with fleshy berry. Accordingly, it is very important to monitor and to increase the vitamin C content in tomato fruit, for meeting the consumer demand and health requirements for high nutrition, e.g. by overexpressing D-galacturonate reductase
Fragaria x ananassa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene GalUR, recombinant overexpression in Solanum lycopersicum cv. Ailsa Craig leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection resulting in 2fold and 1.6fold increase in ascorbate level in tomato fruit and leaf, respectively, which correlates positively with FaGalUR transcriptional abundance and enzyme GalUR activity compared to wild-type plants, real-time PCR expression analysis
Fragaria x ananassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Fragaria x ananassa
NADPH
-
Fragaria x ananassa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
gene FaGalUR-overexpressing Solanum lycopersicum plants show enhanced ascorbic acid levels, tolerance to abiotic stresses induced by oxidization (methyl viologen), salt (NaCl), and cold compared to the wild-type plants, overview. Ascorbate accumulation in tomato can be enhanced by regulating Fragaria x ananassa GalUR gene
Fragaria x ananassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Fragaria x ananassa
-
L-galactonate + NADP+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Fragaria x ananassa
-
leaf
-
Fragaria x ananassa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
743465
Fragaria x ananassa
L-galactonate + NADP+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Fragaria x ananassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Fragaria x ananassa
General Information
General Information
Commentary
Organism
physiological function
the D-galacturonic acid reductase catalyzes the conversion of D-galacturonic acid to L-galactonic acid in plant. The D-galacturonic acid, known as an abundant component of the cell wall, is a degradation product of pectin in senescencing plant cells. The expression levels of FaGalUR are correlated with increased ascorbate content in ripe strawberry fruit. Fragaria x ananassa gene FaGalUR expression improves salt and cold tolerance in Solanum lycopersicum fruits, and improves tolerance to oxidative stress in tomato
Fragaria x ananassa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the D-galacturonic acid reductase catalyzes the conversion of D-galacturonic acid to L-galactonic acid in plant. The D-galacturonic acid, known as an abundant component of the cell wall, is a degradation product of pectin in senescencing plant cells. The expression levels of FaGalUR are correlated with increased ascorbate content in ripe strawberry fruit. Fragaria x ananassa gene FaGalUR expression improves salt and cold tolerance in Solanum lycopersicum fruits, and improves tolerance to oxidative stress in tomato
Fragaria x ananassa
Other publictions for EC 1.1.1.365
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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743465
Cai
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Ectopic expression of FaGalUR ...
Fragaria x ananassa
Plant Growth Regul.
76
187-197
2015
-
1
1
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1
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1
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1
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2
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1
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1
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741576
Sloothaak
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Aspergillus niger, Aspergillus niger CBS 120.49
AMB Express
4
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1
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1
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1
1
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741553
Yang
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Accumulation of vitamin C and ...
Fragaria x ananassa
Adv. Mater. Res.
749
277-282
2013
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1
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2
2
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721358
Kuivanen
Engineering filamentous fungi ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei
Appl. Environ. Microbiol.
78
8676-8683
2012
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9
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725116
Venkatesh
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Chlorophyll a fluorescence tra ...
Fragaria x ananassa
Hortic. Environ. Biotechnol.
53
320-328
2012
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715098
Zhang
The D-galacturonic acid catabo ...
Botrytis cinerea, Botrytis cinerea B05.10
Fungal Genet. Biol.
48
990-997
2011
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1
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4
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2
2
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14
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1
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1
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1
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1
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725952
Hemavath
-
Biochemical analysis of enhanc ...
Fragaria x ananassa
Mol. Breed.
28
105-115
2011
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1
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723997
Mojzita
Metabolic engineering of funga ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
76
169-175
2010
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14
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2
2
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726236
Hemavath
-
Over-expression of strawberry ...
Fragaria x ananassa
Plant Sci.
177
659-667
2009
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1
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1
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722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger, Aspergillus niger CBS 120.49
Fungal Genet. Biol.
45
1449-1457
2008
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1
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6
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1
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8
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4
4
724580
Ishikawa
Functional characterization of ...
Euglena gracilis, Euglena gracilis Z
Biosci. Biotechnol. Biochem.
70
2720-2726
2006
2
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4
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2
2
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22
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16
1
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3
1
1
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1
1
1
1
3
3
724270
Kuorelahti
Identification in the mold Hyp ...
Trichoderma reesei, Trichoderma reesei RUT C-30
Biochemistry
44
11234-11240
2005
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1
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6
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13
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10
1
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726022
Agius
Engineering increased vitamin ...
Fragaria x ananassa
Nat. Biotechnol.
21
177-181
2003
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1
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1
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