BRENDA - Enzyme Database
show all sequences of 1.1.1.365

Increased antioxidant capacity in tomato by ectopic expression of the strawberry D-galacturonate reductase gene

Amaya, I.; Osorio, S.; Martinez-Ferri, E.; Lima-Silva, V.; Doblas, V.G.; Fernandez-Munoz, R.; Fernie, A.R.; Botella, M.A.; Valpuesta, V.; Biotechnol. J. 10, 490-500 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
ORF FaGalUR is recombinantly expressed in Solanum lycopersicum from plasmid pPGGUSI, which contains a 4.8 Kb promoter fragment of the polygalacturonase (PG) gene and its terminator, or from a plasmid containing the 35S CaMV promoter, mobilized from Escherichia coli by triparental mating, via Agrobacterium tumefaciens strain LBA4404 in tomato plants, PCR expression analysis. FaGalUR expression driven by the CaMV 35S promoter causes the expression of the gene in most parts of the plants, while in the second, FaGalU, is expressed under the control of the tomato fruit ripening-specific PG promoter. All of the transgenic lines are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. No significant changes in soluble solids of transgenic plants, but a reduction in acidity
Fragaria x ananassa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fragaria x ananassa
O49133
Fragaria chiloensis x Fragaria virginiana
-
Cloned(Commentary) (protein specific)
Commentary
Organism
ORF FaGalUR is recombinantly expressed in Solanum lycopersicum from plasmid pPGGUSI, which contains a 4.8 Kb promoter fragment of the polygalacturonase (PG) gene and its terminator, or from a plasmid containing the 35S CaMV promoter, mobilized from Escherichia coli by triparental mating, via Agrobacterium tumefaciens strain LBA4404 in tomato plants, PCR expression analysis. FaGalUR expression driven by the CaMV 35S promoter causes the expression of the gene in most parts of the plants, while in the second, FaGalU, is expressed under the control of the tomato fruit ripening-specific PG promoter. All of the transgenic lines are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. No significant changes in soluble solids of transgenic plants, but a reduction in acidity
Fragaria x ananassa
General Information
General Information
Commentary
Organism
physiological function
all of transgenic Solanum lycopersicum lines overexpressing the Fragaria ananassa D-galacturonic acid reductase gene are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. The plants show no significant changes in soluble solids of transgenic plants, but a reduction in acidity. Transgenic lines show a moderate increase on AsA content, and complex changes in metabolites are found in transgenic fruits. Phenotypes, overview
Fragaria x ananassa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
all of transgenic Solanum lycopersicum lines overexpressing the Fragaria ananassa D-galacturonic acid reductase gene are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. The plants show no significant changes in soluble solids of transgenic plants, but a reduction in acidity. Transgenic lines show a moderate increase on AsA content, and complex changes in metabolites are found in transgenic fruits. Phenotypes, overview
Fragaria x ananassa
Other publictions for EC 1.1.1.365
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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743438
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Transgenic tomato plants expr ...
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Plant Biotechnol. Rep.
10
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2016
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Increased antioxidant capacit ...
Fragaria x ananassa
Biotechnol. J.
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490-500
2015
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743465
Cai
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Ectopic expression of FaGalUR ...
Fragaria x ananassa
Plant Growth Regul.
76
187-197
2015
-
1
1
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1
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741576
Sloothaak
Overexpression of the Aspergi ...
Aspergillus niger, Aspergillus niger CBS 120.49
AMB Express
4
66
2014
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1
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741553
Yang
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Accumulation of vitamin C and ...
Fragaria x ananassa
Adv. Mater. Res.
749
277-282
2013
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721358
Kuivanen
Engineering filamentous fungi ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei
Appl. Environ. Microbiol.
78
8676-8683
2012
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9
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725116
Venkatesh
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Chlorophyll a fluorescence tra ...
Fragaria x ananassa
Hortic. Environ. Biotechnol.
53
320-328
2012
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715098
Zhang
The D-galacturonic acid catabo ...
Botrytis cinerea, Botrytis cinerea B05.10
Fungal Genet. Biol.
48
990-997
2011
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1
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4
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14
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725952
Hemavath
-
Biochemical analysis of enhanc ...
Fragaria x ananassa
Mol. Breed.
28
105-115
2011
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723997
Mojzita
Metabolic engineering of funga ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
76
169-175
2010
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2
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726236
Hemavath
-
Over-expression of strawberry ...
Fragaria x ananassa
Plant Sci.
177
659-667
2009
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1
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722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger, Aspergillus niger CBS 120.49
Fungal Genet. Biol.
45
1449-1457
2008
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1
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8
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4
4
724580
Ishikawa
Functional characterization of ...
Euglena gracilis, Euglena gracilis Z
Biosci. Biotechnol. Biochem.
70
2720-2726
2006
2
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1
4
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2
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22
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16
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1
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1
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3
3
724270
Kuorelahti
Identification in the mold Hyp ...
Trichoderma reesei, Trichoderma reesei RUT C-30
Biochemistry
44
11234-11240
2005
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6
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13
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10
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726022
Agius
Engineering increased vitamin ...
Fragaria x ananassa
Nat. Biotechnol.
21
177-181
2003
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