BRENDA - Enzyme Database
show all sequences of 1.1.1.365

Accumulation of vitamin C and enhanced tolerance to iron deficiency stress in transgenic tomato with GalUR gene

Yang, J.; Liu, Y.; Huang, J.; Li, J.; Shi, Y.; Liu, Y.; Adv. Mater. Res. 749, 277-282 (2013)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene GalUR, recombinant stable overexpression in Solanum lycopersicum cv. Zhongshu 4 leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection method using the pCB302-3 vector and CaMV 35S promoter, reverse transcriptase-PCR expression analysis. The transgenic plants show 2.2-2.5fold increased enzyme activity compared to wild-type
Fragaria x ananassa
Engineering
Amino acid exchange
Commentary
Organism
additional information
stable overexpressing the Fragaria GalUR gene results in 2.6fold increase of ascorbate in fruits and 1.6fold increase of ascorbate in leaves compared to non-transformed wild-type tomato, the levels of ascorbate are positively correlated with increased GalUR activity. The transgenic plants show enhanced tolerance to iron deficiency compared to wild-type plants. Under Fe(II) deficiency condition the plant height of transgenic plants is 1.2-1.7times higher, the ascorbate content is 1.8-2.8times higher, and the Fe2+ content is 1.1-1.4times higher compared to wild-type
Fragaria x ananassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Fragaria x ananassa
-
L-galactonate + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fragaria x ananassa
O49133
Fragaria chiloensis x Fragaria virginiana
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Fragaria x ananassa
-
leaf
-
Fragaria x ananassa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
741553
Fragaria x ananassa
L-galactonate + NADP+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Fragaria x ananassa
NADPH
-
Fragaria x ananassa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene GalUR, recombinant stable overexpression in Solanum lycopersicum cv. Zhongshu 4 leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection method using the pCB302-3 vector and CaMV 35S promoter, reverse transcriptase-PCR expression analysis. The transgenic plants show 2.2-2.5fold increased enzyme activity compared to wild-type
Fragaria x ananassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Fragaria x ananassa
NADPH
-
Fragaria x ananassa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
stable overexpressing the Fragaria GalUR gene results in 2.6fold increase of ascorbate in fruits and 1.6fold increase of ascorbate in leaves compared to non-transformed wild-type tomato, the levels of ascorbate are positively correlated with increased GalUR activity. The transgenic plants show enhanced tolerance to iron deficiency compared to wild-type plants. Under Fe(II) deficiency condition the plant height of transgenic plants is 1.2-1.7times higher, the ascorbate content is 1.8-2.8times higher, and the Fe2+ content is 1.1-1.4times higher compared to wild-type
Fragaria x ananassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Fragaria x ananassa
-
L-galactonate + NADP+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Fragaria x ananassa
-
leaf
-
Fragaria x ananassa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
741553
Fragaria x ananassa
L-galactonate + NADP+
-
-
-
r
General Information
General Information
Commentary
Organism
metabolism
D-galacturonate reductase (GalUR) is important in the ascorbic acid biosynthetic pathway
Fragaria x ananassa
physiological function
D-galacturonate reductase (GalUR) plays a prominent role in the regulation of the ascorbate biosynthetic pathway. The overexpression of gene GalUR gene enhances the level of ascorbate and Fe(II) of transgenic tomato plants which show better growth than wild-type plants under iron stresses. Ascorbate is a cofactor for many enzymes and affects the expression of genes involved in defense signaling pathways. It plays an important role as an antioxidant and protects the plant during oxidative damage by scavenging free radicals and reactive oxygen species that are generated during photosynthesis, oxidative metabolism and various abiotic stresses including excess light, soil water stress, UV-B radiation, and ozone
Fragaria x ananassa
General Information (protein specific)
General Information
Commentary
Organism
metabolism
D-galacturonate reductase (GalUR) is important in the ascorbic acid biosynthetic pathway
Fragaria x ananassa
physiological function
D-galacturonate reductase (GalUR) plays a prominent role in the regulation of the ascorbate biosynthetic pathway. The overexpression of gene GalUR gene enhances the level of ascorbate and Fe(II) of transgenic tomato plants which show better growth than wild-type plants under iron stresses. Ascorbate is a cofactor for many enzymes and affects the expression of genes involved in defense signaling pathways. It plays an important role as an antioxidant and protects the plant during oxidative damage by scavenging free radicals and reactive oxygen species that are generated during photosynthesis, oxidative metabolism and various abiotic stresses including excess light, soil water stress, UV-B radiation, and ozone
Fragaria x ananassa
Other publictions for EC 1.1.1.365
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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743465
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Fragaria x ananassa
Plant Growth Regul.
76
187-197
2015
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1
1
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1
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1
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741576
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Aspergillus niger, Aspergillus niger CBS 120.49
AMB Express
4
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741553
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-
Accumulation of vitamin C and ...
Fragaria x ananassa
Adv. Mater. Res.
749
277-282
2013
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1
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2
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Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei
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725116
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715098
Zhang
The D-galacturonic acid catabo ...
Botrytis cinerea, Botrytis cinerea B05.10
Fungal Genet. Biol.
48
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2011
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1
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4
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2
2
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14
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1
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1
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1
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1
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725952
Hemavath
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Biochemical analysis of enhanc ...
Fragaria x ananassa
Mol. Breed.
28
105-115
2011
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1
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Mojzita
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Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
76
169-175
2010
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2
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726236
Hemavath
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Over-expression of strawberry ...
Fragaria x ananassa
Plant Sci.
177
659-667
2009
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722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger, Aspergillus niger CBS 120.49
Fungal Genet. Biol.
45
1449-1457
2008
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6
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1
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4
4
724580
Ishikawa
Functional characterization of ...
Euglena gracilis, Euglena gracilis Z
Biosci. Biotechnol. Biochem.
70
2720-2726
2006
2
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3
3
724270
Kuorelahti
Identification in the mold Hyp ...
Trichoderma reesei, Trichoderma reesei RUT C-30
Biochemistry
44
11234-11240
2005
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726022
Agius
Engineering increased vitamin ...
Fragaria x ananassa
Nat. Biotechnol.
21
177-181
2003
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