BRENDA - Enzyme Database
show all sequences of 1.1.1.365

Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase

Agius, F.; Gonzalez-Lamothe, R.; Caballero, J.L.; Munoz-Blanco, J.; Botella, M.A.; Valpuesta, V.; Nat. Biotechnol. 21, 177-181 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Arabidopsis thaliana and in Escherichia coli XL1-Blue MRF' cells
Fragaria x ananassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Fragaria x ananassa
-
L-galactonate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fragaria x ananassa
-
-
-
Purification (Commentary)
Commentary
Organism
glutathione agarose column chromatography
Fragaria x ananassa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Fragaria x ananassa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
726022
Fragaria x ananassa
L-galactonate + NADP+
-
-
-
?
D-glucuronate + NADPH + H+
-
726022
Fragaria x ananassa
?
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Fragaria x ananassa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Arabidopsis thaliana and in Escherichia coli XL1-Blue MRF' cells
Fragaria x ananassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Fragaria x ananassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Fragaria x ananassa
-
L-galactonate + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione agarose column chromatography
Fragaria x ananassa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Fragaria x ananassa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
726022
Fragaria x ananassa
L-galactonate + NADP+
-
-
-
?
D-glucuronate + NADPH + H+
-
726022
Fragaria x ananassa
?
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
enzyme expression correlates with changing ascorbic acid content in strawberry fruit during ripening. Overexpression of the enzyme in Arabidopsis thaliana enhances vitamin C content 2-3fold
Fragaria x ananassa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme expression correlates with changing ascorbic acid content in strawberry fruit during ripening. Overexpression of the enzyme in Arabidopsis thaliana enhances vitamin C content 2-3fold
Fragaria x ananassa
Other publictions for EC 1.1.1.365
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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1
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2
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1
2
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1
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2
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1
-
1
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1
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2
2
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1
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1
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1
2
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1
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4
1
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1
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1
1
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743438
Lim
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Transgenic tomato plants expr ...
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Plant Biotechnol. Rep.
10
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2016
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1
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2
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2
-
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-
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-
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-
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-
742163
Amaya
Increased antioxidant capacit ...
Fragaria x ananassa
Biotechnol. J.
10
490-500
2015
-
-
1
-
-
-
-
-
-
-
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-
4
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
1
1
-
-
-
743465
Cai
-
Ectopic expression of FaGalUR ...
Fragaria x ananassa
Plant Growth Regul.
76
187-197
2015
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
741576
Sloothaak
Overexpression of the Aspergi ...
Aspergillus niger, Aspergillus niger CBS 120.49
AMB Express
4
66
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
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-
-
1
-
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2
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1
2
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2
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-
1
-
-
2
-
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-
1
-
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-
1
1
-
-
-
741553
Yang
-
Accumulation of vitamin C and ...
Fragaria x ananassa
Adv. Mater. Res.
749
277-282
2013
-
-
1
-
1
-
-
-
-
-
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1
-
1
-
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-
2
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-
1
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2
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1
2
-
1
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-
1
-
-
-
-
-
2
-
-
1
-
-
-
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-
-
-
-
-
-
2
2
-
-
-
721358
Kuivanen
Engineering filamentous fungi ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei
Appl. Environ. Microbiol.
78
8676-8683
2012
-
-
-
-
-
-
-
-
-
-
-
3
-
9
-
-
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-
-
-
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-
3
-
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-
-
-
-
-
-
2
-
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-
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2
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3
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3
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-
-
-
-
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-
-
-
-
-
725116
Venkatesh
-
Chlorophyll a fluorescence tra ...
Fragaria x ananassa
Hortic. Environ. Biotechnol.
53
320-328
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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1
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-
-
715098
Zhang
The D-galacturonic acid catabo ...
Botrytis cinerea, Botrytis cinerea B05.10
Fungal Genet. Biol.
48
990-997
2011
-
-
1
-
-
-
-
4
-
-
2
2
-
14
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
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1
1
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4
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2
2
-
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-
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-
4
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
725952
Hemavath
-
Biochemical analysis of enhanc ...
Fragaria x ananassa
Mol. Breed.
28
105-115
2011
-
-
1
-
-
-
-
-
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1
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1
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-
723997
Mojzita
Metabolic engineering of funga ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
76
169-175
2010
-
-
-
-
-
-
-
-
-
-
-
4
-
14
-
-
-
-
-
2
-
-
4
-
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-
-
-
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-
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2
-
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2
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4
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2
-
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4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726236
Hemavath
-
Over-expression of strawberry ...
Fragaria x ananassa
Plant Sci.
177
659-667
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
1
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-
1
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1
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1
1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger, Aspergillus niger CBS 120.49
Fungal Genet. Biol.
45
1449-1457
2008
-
-
1
-
-
-
-
4
-
-
1
2
-
7
-
-
-
-
-
1
6
-
12
1
-
-
-
8
-
-
-
2
-
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-
1
2
-
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-
4
-
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1
2
-
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-
1
6
-
12
1
-
-
-
8
-
-
-
-
-
-
-
-
4
4
724580
Ishikawa
Functional characterization of ...
Euglena gracilis, Euglena gracilis Z
Biosci. Biotechnol. Biochem.
70
2720-2726
2006
2
-
-
-
-
-
1
4
-
2
2
2
-
22
-
-
1
-
-
1
2
-
16
1
-
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3
1
1
-
1
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2
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1
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1
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4
-
2
2
2
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-
1
-
1
2
-
16
1
-
-
-
3
1
1
-
-
1
1
1
1
3
3
724270
Kuorelahti
Identification in the mold Hyp ...
Trichoderma reesei, Trichoderma reesei RUT C-30
Biochemistry
44
11234-11240
2005
-
-
1
-
-
-
-
6
-
-
1
-
-
13
-
-
1
-
-
1
-
-
10
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
6
-
-
1
-
-
-
-
1
-
1
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726022
Agius
Engineering increased vitamin ...
Fragaria x ananassa
Nat. Biotechnol.
21
177-181
2003
-
-
1
-
-
-
-
-
-
-
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1
-
5
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1
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2
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1
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1
1
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1
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1
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1
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2
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1
1
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