BRENDA - Enzyme Database
show all sequences of 1.1.1.365

An evolutionary conserved D-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation

Martens-Uzunova, E.S.; Schaap, P.J.; Fungal Genet. Biol. 45, 1449-1457 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Aspergillus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
NADPH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.175
-
D-galacturonate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.326
-
NADH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
7.11
-
D-galacturonate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
x * 44000, His6-tagged enzyme, SDS-PAGE
Aspergillus niger
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Aspergillus niger
-
L-galactonate + NADP+
-
-
?
D-galacturonate + NADPH + H+
Aspergillus niger CBS 120.49
-
L-galactonate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
-
-
Aspergillus niger CBS 120.49
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus niger
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.12
-
with NAD+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.17
-
with NADH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.21
-
with NADP+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
3.9
-
with NADPH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
6.9
-
with NADH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
8.7
-
with NADPH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADH + H+
-
722316
Aspergillus niger
L-galactonate + NAD+
-
-
-
r
D-galacturonate + NADH + H+
-
722316
Aspergillus niger CBS 120.49
L-galactonate + NAD+
-
-
-
r
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger
L-galactonate + NADP+
-
-
-
?
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger
L-galactonate + NADP+
-
-
-
r
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger CBS 120.49
L-galactonate + NADP+
-
-
-
?
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger CBS 120.49
L-galactonate + NADP+
-
-
-
r
D-glucuronate + NADH + H+
-
722316
Aspergillus niger
?
-
-
-
?
D-glucuronate + NADH + H+
-
722316
Aspergillus niger CBS 120.49
?
-
-
-
?
D-glucuronate + NADPH + H+
-
722316
Aspergillus niger
?
-
-
-
?
D-glucuronate + NADPH + H+
-
722316
Aspergillus niger CBS 120.49
?
-
-
-
?
L-galactonate + NAD+
-
722316
Aspergillus niger
D-galacturonate + NADH + H+
-
-
-
r
L-galactonate + NADP+
-
722316
Aspergillus niger
D-galacturonate + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 44000, His6-tagged enzyme, SDS-PAGE
Aspergillus niger
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.08
-
L-galactonate
with NAD+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.13
-
D-glucuronate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.16
-
L-galactonate
with NADP+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
3.11
-
D-glucuronate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
4.98
-
NADH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
5.45
-
D-galacturonate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
6.88
-
D-galacturonate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
7.11
-
NADPH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Aspergillus niger
NADPH
preferred cofactor
Aspergillus niger
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Aspergillus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Aspergillus niger
NADPH
preferred cofactor
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
NADPH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.175
-
D-galacturonate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.326
-
NADH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
7.11
-
D-galacturonate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
x * 44000, His6-tagged enzyme, SDS-PAGE
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Aspergillus niger
-
L-galactonate + NADP+
-
-
?
D-galacturonate + NADPH + H+
Aspergillus niger CBS 120.49
-
L-galactonate + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus niger
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.12
-
with NAD+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.17
-
with NADH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.21
-
with NADP+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
3.9
-
with NADPH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
6.9
-
with NADH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
8.7
-
with NADPH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADH + H+
-
722316
Aspergillus niger
L-galactonate + NAD+
-
-
-
r
D-galacturonate + NADH + H+
-
722316
Aspergillus niger CBS 120.49
L-galactonate + NAD+
-
-
-
r
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger
L-galactonate + NADP+
-
-
-
?
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger
L-galactonate + NADP+
-
-
-
r
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger CBS 120.49
L-galactonate + NADP+
-
-
-
?
D-galacturonate + NADPH + H+
-
722316
Aspergillus niger CBS 120.49
L-galactonate + NADP+
-
-
-
r
D-glucuronate + NADH + H+
-
722316
Aspergillus niger
?
-
-
-
?
D-glucuronate + NADH + H+
-
722316
Aspergillus niger CBS 120.49
?
-
-
-
?
D-glucuronate + NADPH + H+
-
722316
Aspergillus niger
?
-
-
-
?
D-glucuronate + NADPH + H+
-
722316
Aspergillus niger CBS 120.49
?
-
-
-
?
L-galactonate + NAD+
-
722316
Aspergillus niger
D-galacturonate + NADH + H+
-
-
-
r
L-galactonate + NADP+
-
722316
Aspergillus niger
D-galacturonate + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 44000, His6-tagged enzyme, SDS-PAGE
Aspergillus niger
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.08
-
L-galactonate
with NAD+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.13
-
D-glucuronate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
0.16
-
L-galactonate
with NADP+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
3.11
-
D-glucuronate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
4.98
-
NADH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
5.45
-
D-galacturonate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
6.88
-
D-galacturonate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
7.11
-
NADPH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.767
-
D-galacturonate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
15.72
-
NADH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
39.29
-
D-galacturonate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
197.2
-
NADPH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.767
-
D-galacturonate
with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
15.72
-
NADH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
39.29
-
D-galacturonate
with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
197.2
-
NADPH
with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C
Aspergillus niger
Other publictions for EC 1.1.1.365
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741550
Li
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-
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1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
1
-
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1
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2
-
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1
2
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-
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-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742938
Matsubara
Fermentative production of L- ...
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J. Biosci. Bioeng.
122
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-
-
1
-
1
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
1
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1
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2
-
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-
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-
1
2
-
1
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-
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2
-
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4
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
743438
Lim
-
Transgenic tomato plants expr ...
Fragaria x ananassa
Plant Biotechnol. Rep.
10
105-116
2016
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
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-
2
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1
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1
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2
-
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-
-
-
-
-
-
-
-
-
742163
Amaya
Increased antioxidant capacit ...
Fragaria x ananassa
Biotechnol. J.
10
490-500
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743465
Cai
-
Ectopic expression of FaGalUR ...
Fragaria x ananassa
Plant Growth Regul.
76
187-197
2015
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
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1
-
1
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1
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2
-
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-
1
1
2
-
1
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1
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-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
741576
Sloothaak
Overexpression of the Aspergi ...
Aspergillus niger, Aspergillus niger CBS 120.49
AMB Express
4
66
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
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-
1
2
-
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2
-
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-
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-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
741553
Yang
-
Accumulation of vitamin C and ...
Fragaria x ananassa
Adv. Mater. Res.
749
277-282
2013
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721358
Kuivanen
Engineering filamentous fungi ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei
Appl. Environ. Microbiol.
78
8676-8683
2012
-
-
-
-
-
-
-
-
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3
-
9
-
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-
-
-
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3
-
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2
-
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2
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3
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-
3
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
725116
Venkatesh
-
Chlorophyll a fluorescence tra ...
Fragaria x ananassa
Hortic. Environ. Biotechnol.
53
320-328
2012
-
-
1
-
-
-
-
-
-
-
-
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1
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-
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1
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-
715098
Zhang
The D-galacturonic acid catabo ...
Botrytis cinerea, Botrytis cinerea B05.10
Fungal Genet. Biol.
48
990-997
2011
-
-
1
-
-
-
-
4
-
-
2
2
-
14
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
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-
1
1
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4
-
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2
2
-
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-
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4
1
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-
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1
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1
-
-
725952
Hemavath
-
Biochemical analysis of enhanc ...
Fragaria x ananassa
Mol. Breed.
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105-115
2011
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723997
Mojzita
Metabolic engineering of funga ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
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169-175
2010
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2
2
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726236
Hemavath
-
Over-expression of strawberry ...
Fragaria x ananassa
Plant Sci.
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2009
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1
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722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger, Aspergillus niger CBS 120.49
Fungal Genet. Biol.
45
1449-1457
2008
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6
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1
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8
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4
4
724580
Ishikawa
Functional characterization of ...
Euglena gracilis, Euglena gracilis Z
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2720-2726
2006
2
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3
3
724270
Kuorelahti
Identification in the mold Hyp ...
Trichoderma reesei, Trichoderma reesei RUT C-30
Biochemistry
44
11234-11240
2005
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13
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10
1
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726022
Agius
Engineering increased vitamin ...
Fragaria x ananassa
Nat. Biotechnol.
21
177-181
2003
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