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Literature summary for 1.1.1.365 extracted from

  • Martens-Uzunova, E.S.; Schaap, P.J.
    An evolutionary conserved D-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation (2008), Fungal Genet. Biol., 45, 1449-1457.
    View publication on PubMed
Search for more literature for 1.1.1.365

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 NADPH with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
0.175
-
 D-galacturonate with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
0.326
-
 NADH with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
7.11
-
 D-galacturonate with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, His6-tagged enzyme, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonate + NADPH + H+ Aspergillus niger
-
 L-galactonate + NADP+
-
 ?
D-galacturonate + NADPH + H+ Aspergillus niger CBS 120.49
-
 L-galactonate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger CBS 120.49
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
 with NAD+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
0.17
-
 with NADH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
0.21
-
 with NADP+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
3.9
-
 with NADPH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
6.9
-
 with NADH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
8.7
-
 with NADPH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NADH + H+
-
 Aspergillus niger L-galactonate + NAD+
-
 r
D-galacturonate + NADH + H+
-
 Aspergillus niger CBS 120.49 L-galactonate + NAD+
-
 r
D-galacturonate + NADPH + H+
-
 Aspergillus niger L-galactonate + NADP+
-
 ?
D-galacturonate + NADPH + H+
-
 Aspergillus niger L-galactonate + NADP+
-
 r
D-galacturonate + NADPH + H+
-
 Aspergillus niger CBS 120.49 L-galactonate + NADP+
-
 ?
D-galacturonate + NADPH + H+
-
 Aspergillus niger CBS 120.49 L-galactonate + NADP+
-
 r
D-glucuronate + NADH + H+
-
 Aspergillus niger ?
-
 ?
D-glucuronate + NADH + H+
-
 Aspergillus niger CBS 120.49 ?
-
 ?
D-glucuronate + NADPH + H+
-
 Aspergillus niger ?
-
 ?
D-glucuronate + NADPH + H+
-
 Aspergillus niger CBS 120.49 ?
-
 ?
L-galactonate + NAD+
-
 Aspergillus niger D-galacturonate + NADH + H+
-
 r
L-galactonate + NADP+
-
 Aspergillus niger D-galacturonate + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 44000, His6-tagged enzyme, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
gaaA
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 L-galactonate with NAD+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
0.13
-
 D-glucuronate with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
0.16
-
 L-galactonate with NADP+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
3.11
-
 D-glucuronate with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
4.98
-
 NADH with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
5.45
-
 D-galacturonate with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
6.88
-
 D-galacturonate with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
7.11
-
 NADPH with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Aspergillus niger
NADPH preferred cofactor Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.767
-
 D-galacturonate with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
15.72
-
 NADH with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
39.29
-
 D-galacturonate with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger
197.2
-
 NADPH with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C Aspergillus niger