Cloned (Comment) | Organism |
---|---|
gene zwf-1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) and strain MG1655. The His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADH | competitive inhibition | Pseudomonas putida | |
NADP+ | mixed-type inhibition | Pseudomonas putida | |
NADPH | inhibits competitively when NADP+ is the varying substrate and noncompetitively when D-glucose 6-phosphate is the varying substrate | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and modelling, detailed overview | Pseudomonas putida | |
0.014 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
0.127 | - |
NAD+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
0.946 | - |
D-glucose 6-phosphate | pH 8.0, 30°C, recombinant enzyme, with NADP+ | Pseudomonas putida | |
1.137 | - |
D-glucose 6-phosphate | pH 8.0, 30°C, recombinant enzyme, with NAD+ | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | Pseudomonas putida | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
r | |
D-glucose 6-phosphate + NAD+ | Pseudomonas putida KT 2240 | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
r | |
D-glucose 6-phosphate + NADP+ | Pseudomonas putida | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
D-glucose 6-phosphate + NADP+ | Pseudomonas putida KT 2240 | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
additional information | Pseudomonas putida | the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | A0A1B2F298 | - |
- |
Pseudomonas putida KT 2240 | A0A1B2F298 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ | enzyme PputG6PDH-1 from Pseudomonas putida KT2440 catalyzes the oxidation of D-glucose 6-phosphate using a rapidequilibrium random mechanism | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme | Pseudomonas putida |
0.006 | - |
enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NADP+ | Pseudomonas putida |
0.008 | - |
recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NAD+ | Pseudomonas putida |
0.025 | - |
enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NAD+ | Pseudomonas putida |
0.103 | - |
recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NADP+ | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
Pseudomonas putida | D-glucose 6-phosphate + NAD+ | - |
r | |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
Pseudomonas putida KT 2240 | D-glucose 6-phosphate + NAD+ | - |
r | |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
Pseudomonas putida | D-glucose 6-phosphate + NADP+ | - |
r | |
D-glucose 6-phosphate + NAD+ | - |
Pseudomonas putida | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
r | |
D-glucose 6-phosphate + NAD+ | - |
Pseudomonas putida KT 2240 | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
r | |
D-glucose 6-phosphate + NADP+ | - |
Pseudomonas putida | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
D-glucose 6-phosphate + NADP+ | - |
Pseudomonas putida KT 2240 | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
additional information | the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 | Pseudomonas putida | ? | - |
? | |
additional information | the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate. PputG6PDH-1 is not considered a NADP+-specific enzyme | Pseudomonas putida | ? | - |
? | |
additional information | the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 | Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate. PputG6PDH-1 is not considered a NADP+-specific enzyme | Pseudomonas putida KT 2240 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucose-6-phosphate dehydrogenase | - |
Pseudomonas putida |
PputG6PDH-1 | - |
Pseudomonas putida |
zwf-1 | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
105 | - |
NADPH | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
254 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
277 | - |
NAD+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADH | - |
Pseudomonas putida | |
NADP+ | preferred cofactor | Pseudomonas putida | |
NADPH | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | 0.163 | NADPH | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
0.111 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
1.148 | - |
NAD+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
additional information | the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2181 | - |
NAD+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida | |
7286 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Pseudomonas putida |