Literature summary for 1.1.1.36 extracted from

Qin, Y.M.; Haapalainen, A.M.; Kilpelainen, S.H.; Marttila, M.S.; Koski, M.K.; Glumoff, T.; Novikov, D.K.; Hiltunen, J.K.
Human peroxisomal multifunctional enzyme type 2: site-directed mutagenesis studies show the importance of two protic residues for 2-enoyl-CoA hydratase 2 activity (2000), J. Biol. Chem., 275, 4965-4972.

Search for more literature for 1.1.1.36

Cloned(Commentary)

Cloned (Comment)	Organism
wild type (HsMFE-2) and its variants are expressed in Saccharomyces cerevisiae	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D370A	site-directed mutagenesis	Homo sapiens
D490A	site-directed mutagenesis	Homo sapiens
D510A	site-directed mutagenesis, inactive	Homo sapiens
D517A	site-directed mutagenesis	Homo sapiens
E366A	site-directed mutagenesis, kcat/Km 100times lower than that of the wild type	Homo sapiens
E408A	site-directed mutagenesis	Homo sapiens
G16S	site-directed mutagenesis	Homo sapiens
H406A	site-directed mutagenesis	Homo sapiens
H515A	site-directed mutagenesis	Homo sapiens
H532A	site-directed mutagenesis	Homo sapiens
additional information	constructs are tested for complementation in Saccharomyces cerevisiae	Homo sapiens
Y347A	site-directed mutagenesis	Homo sapiens
Y410A	site-directed mutagenesis	Homo sapiens
Y505A	site-directed mutagenesis	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Homo sapiens	5777	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	alignment of the amino acid sequence of the hydratase 2 domain from human MFE-2 with those of other known hydratase 2 domains from eukaryotes shown	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.023	-	strain HsMFE-2(Y347A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.023	-	strain HsMFE-2(Y410A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.039	-	strain HsMFE-2(E408A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.041	-	strain HsMFE-2(D490A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.046	-	strain HsMFE-2(H406A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.047	-	strain HsMFE-2(D370A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.048	-	strain HsMFE-2(D517A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.051	-	strain HsMFE-2(D510A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.056	-	strain HsMFE-2(E366A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.066	-	strain HsMFE-2(H532A), (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.082	-	strain HsMFE-2, (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens
0.091	-	strain UTL-7A, (3R)-hydroxyacyl-CoA dehydrogenase	Homo sapiens

Synonyms

Synonyms	Comment	Organism
(3R)-hydroxyacyl-CoA dehydrogenase	domain in human MFE-2	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)