Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | above 0.01 mM | Zoogloea ramigera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
acetoacetyl-CoA | cofactor NADPH | Zoogloea ramigera | |
0.02 | 0.021 | NADPH | cosubstrate acetoacetyl-CoA | Zoogloea ramigera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADH + H+ | Zoogloea ramigera | low activity | L-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | Zoogloea ramigera | high activity | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | Zoogloea ramigera | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | Azotobacter beijerinckii | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter beijerinckii | - |
- |
- |
Zoogloea ramigera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, hydroxylapatite, Sephadex G-200 chromatography | Zoogloea ramigera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.7 | - |
NADPH-linked | Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADH + H+ | low activity | Zoogloea ramigera | L-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | - |
Zoogloea ramigera | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | - |
Azotobacter beijerinckii | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | high activity | Zoogloea ramigera | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Zoogloea ramigera | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Azotobacter beijerinckii | D-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Zoogloea ramigera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
reduction of acetoacetyl-CoA | Zoogloea ramigera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Zoogloea ramigera | |
NADH | - |
Azotobacter beijerinckii | |
NADP+ | - |
Zoogloea ramigera | |
NADP+ | - |
Azotobacter beijerinckii | |
NADPH | - |
Zoogloea ramigera | |
NADPH | - |
Azotobacter beijerinckii |