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Literature summary for 1.1.1.357 extracted from

  • Mobus, E.; Maser, E.
    Molecular cloning, overexpression, and characterization of steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. A novel member of the short-chain dehydrogenase/reductase superfamily (1998), J. Biol. Chem., 273, 30888-30896.
    View publication on PubMed
Search for more literature for 1.1.1.357

Cloned(Commentary)

Cloned (Comment) Organism
3alpha-HSD/CR gene cloned into pET15b vector with an N-terminal His tag sequence and transformation of Escherichia coli BL21(DE3) pLysS cells Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
 Fusidic acid pH 8.9, temperature not specified in the publication Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26400
-
 SDS-PAGE Comamonas testosteroni
26400
-
 calculated from deduced amino acid sequence comprising 258 residues Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni P80702
-
-
Comamonas testosteroni ATCC 11996 P80702
-
-

Purification (Commentary)

Purification (Comment) Organism
in one step using metal chelate chromatography, purity of the protein is more than 98% Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fusidic acid + NADP+
-
 Comamonas testosteroni (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NADPH + H+
-
 ?
fusidic acid + NADP+
-
 Comamonas testosteroni ATCC 11996 (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NADPH + H+
-
 ?
additional information no activity with testosterone and progesterone Comamonas testosteroni ?
-
 ?
additional information recombinant 3alpha-HSD/CR protein is active with a variety of C19–27 steroid substrates, catalyzing the 3-oxo reduction and 3alpha-hydroxy dehydrogenation of steroids of the androstane and bile acid series with Km-values in the low micromolar range, 18–42 microM Comamonas testosteroni ?
-
 ?
additional information recombinant enzyme catalyzes carbonyl reduction of nonsteroidal aldehyde and ketone compounds e.g. metyrapone, 4-nitrobenzaldehyde and insecticide NKI 42255, no reverse oxidation of the formed hydroxy derivatives 4-nitrobenzalcohol and NKI 42455 observed Comamonas testosteroni ?
-
 ?
additional information no activity with testosterone and progesterone Comamonas testosteroni ATCC 11996 ?
-
 ?
additional information recombinant 3alpha-HSD/CR protein is active with a variety of C19–27 steroid substrates, catalyzing the 3-oxo reduction and 3alpha-hydroxy dehydrogenation of steroids of the androstane and bile acid series with Km-values in the low micromolar range, 18–42 microM Comamonas testosteroni ATCC 11996 ?
-
 ?
additional information recombinant enzyme catalyzes carbonyl reduction of nonsteroidal aldehyde and ketone compounds e.g. metyrapone, 4-nitrobenzaldehyde and insecticide NKI 42255, no reverse oxidation of the formed hydroxy derivatives 4-nitrobenzalcohol and NKI 42455 observed Comamonas testosteroni ATCC 11996 ?
-
 ?

Synonyms

Synonyms Comment Organism
3alpha-HSD/CR
-
 Comamonas testosteroni
3alpha-hydroxysteroid dehydrogenase/carbonyl reductase
-
 Comamonas testosteroni