BRENDA - Enzyme Database
show all sequences of 1.1.1.353

Participation in aflatoxin biosynthesis by a reductase enzyme encoded by vrdA gene outside the aflatoxin gene cluster

Shima, Y.; Shiina, M.; Shinozawa, T.; Ito, Y.; Nakajima, H.; Adachi, Y.; Yabe, K.; Fungal Genet. Biol. 46, 221-231 (2009) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Aspergillus parasiticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38566
-
x * 38566, calculated from amino acid sequence
Aspergillus parasiticus
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus parasiticus
B9WYE6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus parasiticus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1'-hydroxyversicolorone + NADPH + H+
-
722317
Aspergillus parasiticus
versicolorone + NADP+
-
-
-
r
versiconal + NADPH + H+
-
722317
Aspergillus parasiticus
versiconol + NADP+
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
-
722317
Aspergillus parasiticus
versiconol acetate + NADP+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 38566, calculated from amino acid sequence
Aspergillus parasiticus
Synonyms
Synonyms
Commentary
Organism
VHA reductase II
-
Aspergillus parasiticus
vrdA
-
Aspergillus parasiticus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus parasiticus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Aspergillus parasiticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus parasiticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38566
-
x * 38566, calculated from amino acid sequence
Aspergillus parasiticus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus parasiticus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1'-hydroxyversicolorone + NADPH + H+
-
722317
Aspergillus parasiticus
versicolorone + NADP+
-
-
-
r
versiconal + NADPH + H+
-
722317
Aspergillus parasiticus
versiconol + NADP+
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
-
722317
Aspergillus parasiticus
versiconol acetate + NADP+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38566, calculated from amino acid sequence
Aspergillus parasiticus
General Information
General Information
Commentary
Organism
metabolism
the vrdA is not an aflatoxin biosynthesis gene, although it actually participates in aflatoxin biosynthesis in cells because of its group substrate specificity to intermediates in aflatoxin biosynthesis
Aspergillus parasiticus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the vrdA is not an aflatoxin biosynthesis gene, although it actually participates in aflatoxin biosynthesis in cells because of its group substrate specificity to intermediates in aflatoxin biosynthesis
Aspergillus parasiticus
Other publictions for EC 1.1.1.353
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722317
Shima
Participation in aflatoxin bio ...
Aspergillus parasiticus
Fungal Genet. Biol.
46
221-231
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721332
Yabe
Enzymatic conversion of averuf ...
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Appl. Environ. Microbiol.
69
66-73
2003
-
-
-
-
-
-
-
-
-
-
-
4
-
10
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721319
Matsushima
Purification and characterizat ...
Aspergillus parasiticus, Aspergillus parasiticus NUIH-26
Appl. Environ. Microbiol.
60
2561-2567
1994
-
-
-
-
-
-
-
2
1
-
3
2
-
3
-
-
1
-
-
1
3
-
2
2
2
-
-
-
-
2
1
-
1
-
2
-
-
-
-
1
-
-
-
-
-
-
2
1
-
3
2
-
-
-
1
-
1
3
-
2
2
-
-
-
-
2
1
-
2
-
-
-
-
-
-