Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
apo form, in a binary complex with NADH cofactor, and in a ternary complex with NADH and glyoxylate, hanging drop vapor diffusion method, using 0.1 M MES (pH 6.0) and 4.0 M NaCl, at 22°C | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D141A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
D141E | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
D141N | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
H16A | inactive | Escherichia coli |
H44A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
M251A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
R259A | in the mutant enzyme R259A, the kcat value is approximately 21% that of the wild type enzyme, with about an 11fold increase in Km | Escherichia coli |
R48A | inactive | Escherichia coli |
S140A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
S43A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
Y52F | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
NAD+ | mutant enzyme D141E, pH and temperature not specified in the publication | Escherichia coli | |
0.52 | - |
NAD+ | mutant enzyme D141A, pH and temperature not specified in the publication | Escherichia coli | |
0.56 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
0.65 | - |
NAD+ | mutant enzyme H44A, pH and temperature not specified in the publication | Escherichia coli | |
0.81 | - |
NAD+ | mutant enzyme D141N, pH and temperature not specified in the publication | Escherichia coli | |
0.93 | - |
NAD+ | mutant enzyme M251A, pH and temperature not specified in the publication | Escherichia coli | |
1.06 | - |
(S)-ureidoglycolate | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
1.26 | - |
NAD+ | mutant enzyme Y52F, pH and temperature not specified in the publication | Escherichia coli | |
1.39 | - |
NAD+ | mutant enzyme S140A, pH and temperature not specified in the publication | Escherichia coli | |
1.49 | - |
NAD+ | mutant enzyme R259A, pH and temperature not specified in the publication | Escherichia coli | |
2.28 | - |
NAD+ | mutant enzyme S43A, pH and temperature not specified in the publication | Escherichia coli | |
5.27 | - |
(S)-ureidoglycolate | mutant enzyme D141A, pH and temperature not specified in the publication | Escherichia coli | |
5.84 | - |
(S)-ureidoglycolate | mutant enzyme D141N, pH and temperature not specified in the publication | Escherichia coli | |
10.75 | - |
(S)-ureidoglycolate | mutant enzyme S43A, pH and temperature not specified in the publication | Escherichia coli | |
11.47 | - |
(S)-ureidoglycolate | mutant enzyme R259A, pH and temperature not specified in the publication | Escherichia coli | |
12.38 | - |
(S)-ureidoglycolate | mutant enzyme S140A, pH and temperature not specified in the publication | Escherichia coli | |
13.15 | - |
(S)-ureidoglycolate | mutant enzyme M251A, pH and temperature not specified in the publication | Escherichia coli | |
14.13 | - |
(S)-ureidoglycolate | mutant enzyme D141E, pH and temperature not specified in the publication | Escherichia coli | |
16.82 | - |
(S)-ureidoglycolate | mutant enzyme Y52F, pH and temperature not specified in the publication | Escherichia coli | |
16.94 | - |
(S)-ureidoglycolate | mutant enzyme H44A, pH and temperature not specified in the publication | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-ureidoglycolate + NAD+ | Escherichia coli | - |
oxalurate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77555 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-ureidoglycolate + NAD+ | - |
Escherichia coli | oxalurate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
(S)-ureidoglycolate dehydrogenase | - |
Escherichia coli |
AllD | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | mutant enzyme S140A, pH and temperature not specified in the publication | Escherichia coli | |
0.87 | - |
NAD+ | mutant enzyme D141E, pH and temperature not specified in the publication | Escherichia coli | |
1.03 | - |
NAD+ | mutant enzyme H44A, pH and temperature not specified in the publication | Escherichia coli | |
1.06 | - |
NAD+ | mutant enzyme D141A, pH and temperature not specified in the publication | Escherichia coli | |
1.29 | - |
(S)-ureidoglycolate | mutant enzyme S140A, pH and temperature not specified in the publication | Escherichia coli | |
1.37 | - |
(S)-ureidoglycolate | mutant enzyme S43A, pH and temperature not specified in the publication | Escherichia coli | |
1.47 | - |
NAD+ | mutant enzyme D141N, pH and temperature not specified in the publication | Escherichia coli | |
1.62 | - |
(S)-ureidoglycolate | mutant enzyme D141A, pH and temperature not specified in the publication | Escherichia coli | |
1.67 | - |
(S)-ureidoglycolate | mutant enzyme D141E, pH and temperature not specified in the publication | Escherichia coli | |
1.76 | - |
(S)-ureidoglycolate | mutant enzyme H44A, pH and temperature not specified in the publication | Escherichia coli | |
1.9 | - |
NAD+ | mutant enzyme Y52F, pH and temperature not specified in the publication | Escherichia coli | |
1.92 | - |
(S)-ureidoglycolate | mutant enzyme Y52F, pH and temperature not specified in the publication | Escherichia coli | |
1.97 | - |
(S)-ureidoglycolate | mutant enzyme D141N, pH and temperature not specified in the publication | Escherichia coli | |
2.51 | - |
NAD+ | mutant enzyme S43A, pH and temperature not specified in the publication | Escherichia coli | |
9.29 | - |
NAD+ | mutant enzyme R259A, pH and temperature not specified in the publication | Escherichia coli | |
11.98 | - |
(S)-ureidoglycolate | mutant enzyme R259A, pH and temperature not specified in the publication | Escherichia coli | |
30.65 | - |
NAD+ | mutant enzyme M251A, pH and temperature not specified in the publication | Escherichia coli | |
45.98 | - |
(S)-ureidoglycolate | mutant enzyme M251A, pH and temperature not specified in the publication | Escherichia coli | |
57.06 | - |
(S)-ureidoglycolate | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
62.39 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme selectively utilizes NAD+ as a cofactor | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NAD+ | mutant enzyme S140A, pH and temperature not specified in the publication | Escherichia coli | |
0.1 | - |
(S)-ureidoglycolate | mutant enzyme H44A, pH and temperature not specified in the publication | Escherichia coli | |
0.1 | - |
(S)-ureidoglycolate | mutant enzyme S140A, pH and temperature not specified in the publication | Escherichia coli | |
0.11 | - |
(S)-ureidoglycolate | mutant enzyme Y52F, pH and temperature not specified in the publication | Escherichia coli | |
0.12 | - |
(S)-ureidoglycolate | mutant enzyme D141E, pH and temperature not specified in the publication | Escherichia coli | |
0.13 | - |
(S)-ureidoglycolate | mutant enzyme S43A, pH and temperature not specified in the publication | Escherichia coli | |
0.31 | - |
(S)-ureidoglycolate | mutant enzyme D141A, pH and temperature not specified in the publication | Escherichia coli | |
0.34 | - |
(S)-ureidoglycolate | mutant enzyme D141N, pH and temperature not specified in the publication | Escherichia coli | |
1.04 | - |
(S)-ureidoglycolate | mutant enzyme R259A, pH and temperature not specified in the publication | Escherichia coli | |
1.1 | - |
NAD+ | mutant enzyme S43A, pH and temperature not specified in the publication | Escherichia coli | |
1.51 | - |
NAD+ | mutant enzyme Y52F, pH and temperature not specified in the publication | Escherichia coli | |
1.6 | - |
NAD+ | mutant enzyme H44A, pH and temperature not specified in the publication | Escherichia coli | |
1.81 | - |
NAD+ | mutant enzyme D141N, pH and temperature not specified in the publication | Escherichia coli | |
2.04 | - |
NAD+ | mutant enzyme D141A, pH and temperature not specified in the publication | Escherichia coli | |
2.35 | - |
NAD+ | mutant enzyme D141E, pH and temperature not specified in the publication | Escherichia coli | |
3.49 | - |
(S)-ureidoglycolate | mutant enzyme M251A, pH and temperature not specified in the publication | Escherichia coli | |
6.23 | - |
NAD+ | mutant enzyme R259A, pH and temperature not specified in the publication | Escherichia coli | |
32.9 | - |
NAD+ | mutant enzyme M251A, pH and temperature not specified in the publication | Escherichia coli | |
54.3 | - |
(S)-ureidoglycolate | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
110 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |