Protein Variants | Comment | Organism |
---|---|---|
additional information | no extracellular 3-hydroxyalkanoic acid is produced by wild-type Pseudomonas putida KT2442 or the polyhydroxyalkanoate synthesis operon knockout mutant KTOY01 when lauric acid is used as carbon source. By expressing tesB gene encoding thioesterase II, Pseudomonas putida KT2442 can not produce extracellular 3-hydroxyalkanoic acid monomer, while the polyhydroxyalkanoate operon knockout mutant Pseudomonas putida KTOY01 (pSPD09) produces 0.35 g/l extracellular medium-chain-length 3-hydroxyalkanoic acid. KTOY07 is constructed by further knocking out fadB and fadA genes in Pseudomonas putida KTOY01. As a result, Pseudomonas putida KTOY07 increases medium-chain-length 3-hydroxyalkanoic acid to 1.68 from 0.35 g/l produced by the beta-oxidation intact mutant Pseudomonas putida KTOY01 | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
KT2442, KTOY01 and KTOY07 | - |
Synonyms | Comment | Organism |
---|---|---|
FadB | - |
Pseudomonas putida |