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Literature summary for 1.1.1.35 extracted from
- Direct evidence for the function of FUM13 in 3-ketoreduction of mycotoxin fumonisins in Fusarium verticillioides (2005), J. Agric. Food Chem., 53, 5456-5460.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FUM13, expression of His-tagged enzyme in Escherichia coli, expression in and functional complementation of Saccharomyces cerevisiae 3-ketosphinganine reductase mutant strain tsc10, overview | Fusarium verticillioides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxofumonisin B3 + NADPH | Fusarium verticillioides | the enzyme is required for biosynthesis of fumonisins | fumonisin B3 + NADP+ | - |
? |  |
| 3-oxofumonisin B3 + NADPH | Fusarium verticillioides A0149 | the enzyme is required for biosynthesis of fumonisins | fumonisin B3 + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium verticillioides | - |
gene FUM13 | - |
| Fusarium verticillioides A0149 | - |
gene FUM13 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Fusarium verticillioides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxofumonisin B3 + NADPH | the enzyme is required for biosynthesis of fumonisins | Fusarium verticillioides | fumonisin B3 + NADP+ | - |
? | |
| 3-oxofumonisin B3 + NADPH | 3-keto reduction | Fusarium verticillioides | fumonisin B3 + NADP+ | - |
? | |
| 3-oxofumonisin B3 + NADPH | the enzyme is required for biosynthesis of fumonisins | Fusarium verticillioides A0149 | fumonisin B3 + NADP+ | - |
? | |
| 3-oxofumonisin B3 + NADPH | 3-keto reduction | Fusarium verticillioides A0149 | fumonisin B3 + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fum13p | - |
Fusarium verticillioides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Fusarium verticillioides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Fusarium verticillioides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Fusarium verticillioides | |
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Release 2023.1 (January 2023)
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