Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADH | - |
Sus scrofa | |
0.003 | - |
acetoacetyl-CoA | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Sus scrofa |
66000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | Sus scrofa | - |
acetoacetyl-CoA + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpressed in Escherichia coli, phosphocellulose | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
511 | - |
recombinant enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | - |
Sus scrofa | acetoacetyl-CoA + NADH | - |
r | |
3-ketohexadecanoyl-CoA + NADH | - |
Sus scrofa | (S)-3-hydroxhexadecanoyl-CoA + NAD+ | - |
r | |
3-ketooctanoyl-CoA + NADH | - |
Sus scrofa | (S)-3-hydroxyoctanoyl-CoA + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
3-ketohexadecanoyl-CoA | - |
Sus scrofa | |
102 | - |
3-ketooctanoyl-CoA | - |
Sus scrofa | |
330 | - |
acetoacetyl-CoA | pH 7.0 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa |