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Literature summary for 1.1.1.35 extracted from

  • Barycki, J.J.; O'Brian, L.K.; Bratt, J.M.; Zhang, R.; Sanishvili, R.; Strauss, A.W.; Banaszak, L.J.
    Biochemical characterization and crystal structure determination of human heart short chain L-3-hydroxyacyl-CoA dehydrogenase provide insights into catalytic mechanism (1999), Biochemistry, 38, 5786-5789.
    View publication on PubMed
Search for more literature for 1.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal hexameric histidine tag, expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
50 mM N(2-acetamido)-2-iminodiacetic acid, pH 6.5, polyethylene glycol 4000, 5 mM NAD+ hanging drop, crystals within 3 to 5 days at 18°C, enzyme structure is compromised of two domains, a NAD+-binding domain and a helical C-terminal domain Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0138
-
 acetoacetyl-CoA pH 8.0 Homo sapiens
0.0187
-
 acetoacetyl-CoA pH 7.0 Homo sapiens
0.0345
-
 acetoacetyl-CoA pH 5.0 Homo sapiens
0.045
-
 acetoacetyl-CoA pH 6.0 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Homo sapiens
-
 acetoacetyl-CoA + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1200
-
 His-tagged recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+
-
 Homo sapiens acetoacetyl-CoA + NADH
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
short chain L-3-hydroxyacyl-CoA dehydrogenase displays optimal activity using 6-carbon substrates Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
113
-
 acetoacetyl-CoA pH 8.0 Homo sapiens
155
-
 acetoacetyl-CoA pH 5.0 Homo sapiens
246
-
 acetoacetyl-CoA pH 6.0 Homo sapiens
252
-
 acetoacetyl-CoA pH 7.0 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens