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Literature summary for 1.1.1.348 extracted from
- Conversion of vestitone to medicarpin in alfalfa (Medicago sativa L.) is catalyzed by two independent enzymes. Identification, purification, and characterization of vestitone reductase and 7,2-dihydroxy-4-methoxyisoflavanol dehydratase (1994), J. Biol. Chem., 269, 22372-22378.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | NADPH has no inhibitory effect even at a concentration of 2.5 mM | Medicago sativa | |
| vestitone | vestitone reductase is noticeably inhibited by vestitone concentrations in excess of 0.05 mM, the activity being inhibited over 40% at 0.25 mM vestitone | Medicago sativa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
(3R)-vestitone | in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C | Medicago sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Medicago sativa |
| 38000 | - |
1 * 38000, SDS-PAGE | Medicago sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-vestitone + NADPH + H+ | Medicago sativa | vestitone reductase has strict substrate stereospecificity for (3R)-vestitone | (3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PEG fractionation, Red-agarose column chromatography, MonoQ column chromatography, and DEAE-Sephacel gel filtration | Medicago sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.25 | - |
crude extract, in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C | Medicago sativa |
| 461.1 | - |
after 1844fold purification, in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C | Medicago sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-vestitone + NADPH + H+ | vestitone reductase has strict substrate stereospecificity for (3R)-vestitone | Medicago sativa | (3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, SDS-PAGE | Medicago sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Medicago sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Medicago sativa | |
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Release 2023.1 (January 2023)
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