Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhi |
General Stability | Organism |
---|---|
enzyme is very unstable at low protein concentration | Salmonella enterica subsp. enterica serovar Typhi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine triphosphate ribose | competitive inhibitor with respect to NADPH and either an uncompetitive or a mixed type inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose | Salmonella enterica subsp. enterica serovar Typhi | |
CDP | competitive inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and either an uncompetitive or a mixed type inhibitor with respect to NADPH | Salmonella enterica subsp. enterica serovar Typhi | |
CDP-paratose | competitive inhibitor against CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and mixed type or uncompetitive inhibitor with respect to NADPH | Salmonella enterica subsp. enterica serovar Typhi | |
NADP+ | mixed type or uncompetitive inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and competitive inhibitor for NADPH | Salmonella enterica subsp. enterica serovar Typhi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NADPH | pH 8.0, 25°C | Salmonella enterica subsp. enterica serovar Typhi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
2 * 29000, SDS-PAGE, 2 * 31501, calculated | Salmonella enterica subsp. enterica serovar Typhi |
31501 | - |
2 * 29000, SDS-PAGE, 2 * 31501, calculated | Salmonella enterica subsp. enterica serovar Typhi |
55000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhi | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
93 | - |
pH 8.0, 25°C | Salmonella enterica subsp. enterica serovar Typhi |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 1 year | Salmonella enterica subsp. enterica serovar Typhi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-4-dehydro-3,6-dideoxy-alpha-D-glucose + NADPH + H+ | - |
Salmonella enterica subsp. enterica serovar Typhi | CDP-alpha-D-paratose + NADP+ | conversion is almost quantitative | ? | |
additional information | enzyme catalyzes the stereospecific hydride transfer of the pro-S hydrogen from the C-4' position of the reduced coenzyme to C-4 of the substrate, CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose. The overall equilibrium of this catalysis greatly favors the formation of the reduced sugar product and the oxidized coenzyme | Salmonella enterica subsp. enterica serovar Typhi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29000, SDS-PAGE, 2 * 31501, calculated | Salmonella enterica subsp. enterica serovar Typhi |
Synonyms | Comment | Organism |
---|---|---|
rfbS | - |
Salmonella enterica subsp. enterica serovar Typhi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
buffer 0.2 M potassium phosphate | Salmonella enterica subsp. enterica serovar Typhi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is 10fold prefered over NADH | Salmonella enterica subsp. enterica serovar Typhi | |
NADPH | NADPH is 10fold prefered over NADH. Enzyme exhibits a high affinity for NADPH with a much smaller dissociation constant of 0.005microM compared to the Km of 0.26 mM | Salmonella enterica subsp. enterica serovar Typhi |