Cloned (Comment) | Organism |
---|---|
a truncated protein is expressed in Escherichia coli BL21(DE3) cells | Populus trichocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | Populus trichocarpa | - |
(R)-mevalonate + CoA + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | B9GJZ3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Populus trichocarpa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | highest expression in young leaves | Populus trichocarpa | - |
petiole | lowest expression | Populus trichocarpa | - |
root | - |
Populus trichocarpa | - |
stem | - |
Populus trichocarpa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
Populus trichocarpa | (R)-mevalonate + CoA + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, truncated recombinant enzyme, SDS-PAGE | Populus trichocarpa |
? | x * 61640, calculated from amino acid sequence | Populus trichocarpa |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl-CoA reductase | - |
Populus trichocarpa |
HMGR | - |
Populus trichocarpa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Populus trichocarpa | calculated from amino acid sequence | - |
6.6 |
Organism | Comment | Expression |
---|---|---|
Populus trichocarpa | enzyme expression is increased 6 h after treatment with 0.2 mM abscisic acid, 6 h after treatment with 200 mM NaCl, 3 days after treatment with 10% (w/v) PEG 6000, 1 h after treatment with 2 mM H2O2, and 7 days after cold treatment at 4°C | up |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme overexpression significantly increases abscisic acid, gibberellic acid, carotene, and lycopene content, indicating that the enzyme participates in the regulation of terpenoid compound synthesis | Populus trichocarpa |