Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.34 extracted from

  • Song, A.A.; Abdullah, J.O.; Abdullah, M.P.; Shafee, N.; Othman, R.; Tan, E.F.; Noor, N.M.; Raha, A.R.
    Overexpressing 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) in the lactococcal mevalonate pathway for heterologous plant sesquiterpene production (2012), PLoS ONE, 7, e52444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.34

Application

Application Comment Organism
molecular biology Lactococcus lactis is a potential heterologous host for the production of sesquiterpenes from a herbaceous Malaysian plant, Persicaria minor. A sesquiterpene synthase gene encoding beta-sesquiphellandrene synthase from Persicaria minor is successfully cloned and expressed in Lactococcus lactis. Overexpression of the Lactococcus lactis endogenous 3-hydroxy-3-methylglutaryl Co-A reductase, an established rate-limiting enzyme in the eukaryotic mevalonate pathway, increases the production level of beta-sesquiphellandrene by 1.25-1.60 fold Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the recombinant His-tagged enzyme in Lactococcus lactis strain NZ9000, co-expression with sesquiterpene synthase gene, PMSTS from Persicaria minor incorporated into the pNZ8048 plasmid, with two base substitutions, 796A.G and 1077A.G, introduced into the PMSTS gene during amplification resulting in exchange K266E and a silent mutation Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Lactococcus lactis
-
 (R)-mevalonate + CoA + 2 NADP+
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Lactococcus lactis NZ9000
-
 (R)-mevalonate + CoA + 2 NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis NZ9000
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Lactococcus lactis (R)-mevalonate + CoA + 2 NADP+
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Lactococcus lactis NZ9000 (R)-mevalonate + CoA + 2 NADP+
-
 ?

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl coenzyme A reductase
-
 Lactococcus lactis
HMGR
-
 Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Lactococcus lactis

General Information

General Information Comment Organism
metabolism 3-hydroxy-3-methylglutaryl Co-A reductase is a rate-limiting enzyme in the eukaryotic mevalonate pathway Lactococcus lactis