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Literature summary for 1.1.1.34 extracted from

  • Bochar, D.A.; Brown, J.R.; Doolittle, W.F.; Klenk, H.P.; Lam, W.; Schenk, M.E.; Stauffacher, C.V.; Rodwell, V.W.
    3-Hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic properties of the gene product (1997), J. Bacteriol., 179, 3632-3638.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
lovastatin competitive inhibitor for HMG-CoA binding site Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 3-hydroxy-3-methylglutaryl-CoA
-
 Saccharolobus solfataricus
0.017
-
 3-hydroxy-3-methylglutaryl-CoA pH 5.5, 50°C Saccharolobus solfataricus
0.023
-
 NADPH
-
 Saccharolobus solfataricus
0.023
-
 NADPH pH 5.5, 50°C Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus
-
 (R)-mevalonate + CoA + NADP+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus P2
-
 (R)-mevalonate + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980N1
-
-
Saccharolobus solfataricus P2 Q980N1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus
recombinant enzyme Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.5
-
 50°C, pH 5.5, recombinant enzyme Saccharolobus solfataricus
17500
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + NADPH substrate is (S)-isomer of HMG-CoA Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Saccharolobus solfataricus P2 (R)-mevalonate + CoA + NADP+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + NADPH substrate is (S)-isomer of HMG-CoA Saccharolobus solfataricus P2 (R)-mevalonate + CoA + NADP+
-
 ?
additional information no substrate: NADH Saccharolobus solfataricus ?
-
 ?
additional information no substrate: NADH Saccharolobus solfataricus P2 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 stable Saccharolobus solfataricus
90
-
 half-life at 90°C is 3.2 h Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Saccharolobus solfataricus