BRENDA - Enzyme Database show
show all sequences of 1.1.1.338

Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase

Denger, K.; Cook, A.M.; Microbiology 156, 967-974 (2010)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADPH
pH 9.0, 23°C
Chromohalobacter salexigens
0.14
-
3-sulfopyruvate
pH 9.0, 23°C
Chromohalobacter salexigens
1.2
-
NADP+
pH 9.0, 23°C
Chromohalobacter salexigens
19
-
(S)-3-sulfolactate
pH 9.0, 23°C
Chromohalobacter salexigens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE
Chromohalobacter salexigens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chromohalobacter salexigens
Q1QWN5
DSM 3043
-
Storage Stability
Storage Stability
Organism
-18°C, stable against freezing and thawing
Chromohalobacter salexigens
4°C, stable for several weeks
Chromohalobacter salexigens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-3-sulfolactate + NADP+
specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate
705586
Chromohalobacter salexigens
3-sulfopyruvate + NADPH + H+
-
-
-
r
3-sulfopyruvate + NADPH + H+
specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate
705586
Chromohalobacter salexigens
(S)-3-sulfolactate + NADP+
-
-
-
r
additional information
no substrate: pyruvate, oxaloacetate, (S)-malate, (S)-lactate, (R)-malate, (R)-lactate
705586
Chromohalobacter salexigens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
2 * 37000, SDS-PAGE
Chromohalobacter salexigens
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cosubstrate: NADH
Chromohalobacter salexigens
NADPH
-
Chromohalobacter salexigens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cosubstrate: NADH
Chromohalobacter salexigens
NADPH
-
Chromohalobacter salexigens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADPH
pH 9.0, 23°C
Chromohalobacter salexigens
0.14
-
3-sulfopyruvate
pH 9.0, 23°C
Chromohalobacter salexigens
1.2
-
NADP+
pH 9.0, 23°C
Chromohalobacter salexigens
19
-
(S)-3-sulfolactate
pH 9.0, 23°C
Chromohalobacter salexigens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE
Chromohalobacter salexigens
Storage Stability (protein specific)
Storage Stability
Organism
-18°C, stable against freezing and thawing
Chromohalobacter salexigens
4°C, stable for several weeks
Chromohalobacter salexigens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-3-sulfolactate + NADP+
specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate
705586
Chromohalobacter salexigens
3-sulfopyruvate + NADPH + H+
-
-
-
r
3-sulfopyruvate + NADPH + H+
specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate
705586
Chromohalobacter salexigens
(S)-3-sulfolactate + NADP+
-
-
-
r
additional information
no substrate: pyruvate, oxaloacetate, (S)-malate, (S)-lactate, (R)-malate, (R)-lactate
705586
Chromohalobacter salexigens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
2 * 37000, SDS-PAGE
Chromohalobacter salexigens
Expression
Organism
Commentary
Expression
Chromohalobacter salexigens
expression is induced by growth on sulfolactate
up
Expression (protein specific)
Organism
Commentary
Expression
Chromohalobacter salexigens
expression is induced by growth on sulfolactate
up
Other publictions for EC 1.1.1.338
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705586
Denger
Racemase activity effected by ...
Chromohalobacter salexigens
Microbiology
156
967-974
2010
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