Cloned (Comment) | Organism |
---|---|
gene zsd1, recombinant expression in Saccharomyces cerevisiae strain W303-1a, functional co-expression with alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1), and AtCPR1 from Arabidopsis thaliana, subcloning in Escherichia coli | Zingiber zerumbet |
Protein Variants | Comment | Organism |
---|---|---|
additional information | de novo production of zerumbone is achieved in a metabolically engineered yeast cell factory by introducing alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1), and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana into a Saccharomyces cerevisiae strain LW14-16. The overexpression of the mevalonate (MVA) pathway rate-limiting enzymes tHMG1 and ERG20, regulation of ERG9 by an inducible promoter and competitive pathway deletion redirects the metabolic flux toward the desired product. Expressing ZSD1S114A in the ura3 site of strain LW14 results in the production of 7 mg/l zerumbone, multicopy integration of ZSD1S114A increases the production of zerumbone to 20.6 mg/l, production of zerumbone finally reached 40 mg/l by fed-batch fermentation in a 5-l bioreactor using strain LW16, method overview | Zingiber zerumbet |
S114A | site-directed mutagenesis, the mutation of Ser144 to Ala enhances the catalytic efficiency of ZSD1 to 10-hydroxy-alpha-humulene compared to wild-type | Zingiber zerumbet |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-hydroxy-alpha-humulene + NAD+ | Zingiber zerumbet | - |
zerumbone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zingiber zerumbet | F1SWA0 | Amomum zerumbet | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-hydroxy-alpha-humulene + NAD+ | - |
Zingiber zerumbet | zerumbone + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ZSD1 | - |
Zingiber zerumbet |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zingiber zerumbet | |
NADH | - |
Zingiber zerumbet |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the short-chain dehydrogenase enzyme superfamily | Zingiber zerumbet |
physiological function | the enzyme catalyzes the biosynthesis of zerumbone, a monocyclic sesquiterpenoid ketone with potential anti-inflammatory and anti-carcinogenic effects and also potential applications in obesity prevention and treatment | Zingiber zerumbet |