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Literature summary for 1.1.1.326 extracted from

  • Zhang, C.; Liu, J.; Zhao, F.; Lu, C.; Zhao, G.R.; Lu, W.
    Production of sesquiterpenoid zerumbone from metabolic engineered Saccharomyces cerevisiae (2018), Metab. Eng., 49, 28-35 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene zsd1, recombinant expression in Saccharomyces cerevisiae strain W303-1a, functional co-expression with alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1), and AtCPR1 from Arabidopsis thaliana, subcloning in Escherichia coli Zingiber zerumbet

Protein Variants

Protein Variants Comment Organism
additional information de novo production of zerumbone is achieved in a metabolically engineered yeast cell factory by introducing alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1), and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana into a Saccharomyces cerevisiae strain LW14-16. The overexpression of the mevalonate (MVA) pathway rate-limiting enzymes tHMG1 and ERG20, regulation of ERG9 by an inducible promoter and competitive pathway deletion redirects the metabolic flux toward the desired product. Expressing ZSD1S114A in the ura3 site of strain LW14 results in the production of 7 mg/l zerumbone, multicopy integration of ZSD1S114A increases the production of zerumbone to 20.6 mg/l, production of zerumbone finally reached 40 mg/l by fed-batch fermentation in a 5-l bioreactor using strain LW16, method overview Zingiber zerumbet
S114A site-directed mutagenesis, the mutation of Ser144 to Ala enhances the catalytic efficiency of ZSD1 to 10-hydroxy-alpha-humulene compared to wild-type Zingiber zerumbet

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-hydroxy-alpha-humulene + NAD+ Zingiber zerumbet
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zerumbone + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Zingiber zerumbet F1SWA0 Amomum zerumbet
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-hydroxy-alpha-humulene + NAD+
-
Zingiber zerumbet zerumbone + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
ZSD1
-
Zingiber zerumbet

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Zingiber zerumbet
NADH
-
Zingiber zerumbet

General Information

General Information Comment Organism
evolution the enzyme belongs to the short-chain dehydrogenase enzyme superfamily Zingiber zerumbet
physiological function the enzyme catalyzes the biosynthesis of zerumbone, a monocyclic sesquiterpenoid ketone with potential anti-inflammatory and anti-carcinogenic effects and also potential applications in obesity prevention and treatment Zingiber zerumbet